Gene transfection-mediated overexpression of β1,4-N-acetylglucosamine bisecting oligosaccharides in glioma cell line U373 MG inhibits epidermal growth factor receptor function

Abdelhadi Rebbaa, Hirotaka Yamamoto, Tasuku Saito, Emmanuelle Meuillet, Peter Kim, Donna S. Kersey, Eric G. Bremer, Naoyuki Taniguchi, Joseph R. Moskal

Research output: Contribution to journalArticle

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Abstract

N-linked oligosaccharides appear to be important for the function of the epidermal growth factor (EGF) receptor. In a previous study (Rebbaa, A., Yamamoto, H., Moskal, J. R., and Bremer, E. G. (1996) J. Neurochem. 67, 2265- 2272), we showed that binding of the erythroagglutinating phytohemagglutin lectin from Phaseolus vulgaris to the bisecting structures on the EGF receptor from U373 MG glioma cells blocked EGF binding and receptor autophosphorylation. In this study we examined the consequences of overexpression of the bisecting structure on the EGF receptor by gene transfection of U373 MG cells with the N-acetylglucosaminyltransferase III (GnT-III). This modification leads to a significant decrease in EGF binding and EGF receptor autophosphorylation. In addition, the cellular response to EGF was found to be altered. Proliferation of U373 MG cells in serum-free medium is inhibited by EGF. In contrast, proliferation of the GnT-III- transfected cells was stimulated by EGF. These data demonstrate that changes in EGF receptor glycosylation by GnT-III transfection reduces the number of the active receptors in U373 MG cells and that this change results in change in the cellular response to EGF.

Original languageEnglish (US)
Pages (from-to)9275-9279
Number of pages5
JournalJournal of Biological Chemistry
Volume272
Issue number14
DOIs
StatePublished - Apr 4 1997
Externally publishedYes

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Acetylglucosamine
Oligosaccharides
Epidermal Growth Factor Receptor
Epidermal Growth Factor
Glioma
Transfection
Genes
Cells
Cell Line
beta-1,4-mannosyl-glycoprotein beta-1,4-N-acetylglucosaminyltransferase
erbB-1 Genes
Glycosylation
Serum-Free Culture Media
Phytohemagglutinins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Gene transfection-mediated overexpression of β1,4-N-acetylglucosamine bisecting oligosaccharides in glioma cell line U373 MG inhibits epidermal growth factor receptor function. / Rebbaa, Abdelhadi; Yamamoto, Hirotaka; Saito, Tasuku; Meuillet, Emmanuelle; Kim, Peter; Kersey, Donna S.; Bremer, Eric G.; Taniguchi, Naoyuki; Moskal, Joseph R.

In: Journal of Biological Chemistry, Vol. 272, No. 14, 04.04.1997, p. 9275-9279.

Research output: Contribution to journalArticle

Rebbaa, Abdelhadi ; Yamamoto, Hirotaka ; Saito, Tasuku ; Meuillet, Emmanuelle ; Kim, Peter ; Kersey, Donna S. ; Bremer, Eric G. ; Taniguchi, Naoyuki ; Moskal, Joseph R. / Gene transfection-mediated overexpression of β1,4-N-acetylglucosamine bisecting oligosaccharides in glioma cell line U373 MG inhibits epidermal growth factor receptor function. In: Journal of Biological Chemistry. 1997 ; Vol. 272, No. 14. pp. 9275-9279.
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