Generation of a cytotoxic T-lymphocyte response using a Salmonella antigen-delivery system

J. L. Flynn, W. R. Weiss, K. A. Norris, H. S. Seifert, S. Kumar, Magdalene "Maggie" So

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

We have constructed a general-use vector for the cloning and stable expression of foreign genes in the chromosome of attenuated Salmonella typhimurium. Using this chromosomal expression vector (CEV), we expressed the circumsporozoite (CS) gene of the mouse malaria Plasmodium yoelii in an aroA S. typhimurium strain. Mice immunized with CS-expressing Salmonella recombinants mount a CS-specific cytotoxic T-lymphocyte (CTL) response. This is the first demonstration that attenuated Salmonella can elicit a specific CTL response to a foreign protein in mice. The ability to easily and stably express foreign genes from the Salmonella chromosome and the generation of specific CTL greatly expands the potential of Salmonella as an antigen-delivery system.

Original languageEnglish (US)
Pages (from-to)2111-2118
Number of pages8
JournalMolecular Microbiology
Volume4
Issue number12
StatePublished - Dec 1990
Externally publishedYes

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Cytotoxic T-Lymphocytes
Salmonella
Antigens
Salmonella typhimurium
Chromosomes
Plasmodium yoelii
Genetic Vectors
Genes
Malaria
Gene Expression
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

Cite this

Flynn, J. L., Weiss, W. R., Norris, K. A., Seifert, H. S., Kumar, S., & So, M. M. (1990). Generation of a cytotoxic T-lymphocyte response using a Salmonella antigen-delivery system. Molecular Microbiology, 4(12), 2111-2118.

Generation of a cytotoxic T-lymphocyte response using a Salmonella antigen-delivery system. / Flynn, J. L.; Weiss, W. R.; Norris, K. A.; Seifert, H. S.; Kumar, S.; So, Magdalene "Maggie".

In: Molecular Microbiology, Vol. 4, No. 12, 12.1990, p. 2111-2118.

Research output: Contribution to journalArticle

Flynn, JL, Weiss, WR, Norris, KA, Seifert, HS, Kumar, S & So, MM 1990, 'Generation of a cytotoxic T-lymphocyte response using a Salmonella antigen-delivery system', Molecular Microbiology, vol. 4, no. 12, pp. 2111-2118.
Flynn JL, Weiss WR, Norris KA, Seifert HS, Kumar S, So MM. Generation of a cytotoxic T-lymphocyte response using a Salmonella antigen-delivery system. Molecular Microbiology. 1990 Dec;4(12):2111-2118.
Flynn, J. L. ; Weiss, W. R. ; Norris, K. A. ; Seifert, H. S. ; Kumar, S. ; So, Magdalene "Maggie". / Generation of a cytotoxic T-lymphocyte response using a Salmonella antigen-delivery system. In: Molecular Microbiology. 1990 ; Vol. 4, No. 12. pp. 2111-2118.
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