Generation of a cytotoxic T‐lymphocyte response using a Salmonella antigen‐delivery system

J. L. Flynn, W. R. Weiss, K. A. Norris, H. S. Seifert, S. Kumar, M. So

Research output: Contribution to journalArticle

86 Scopus citations

Abstract

We have constructed a general‐use vector for the cloning and stable expression of foreign genes in the chromosome of attenuated Salmonella typhimurium. Using this chromosomal expression vector (CEV), we expressed the circumsporozoite (CS) gene of the mouse malaria Plasmodium yoelii in an aroA S. typhimurium strain. Mice immunized with CS‐expressing Salmonella recombinants mount a CS‐specific cytotoxic T‐lymphocyte (CTL) response. This is the first demonstration that attenuated Salmonella can elicit a specific CTL response to a foreign protein in mice. The ability to easily and stably express foreign genes from the Salmonella chromosome and the generation of specific CTL greatly expands the potential of Salmonella as an antigen‐delivery system.

Original languageEnglish (US)
Pages (from-to)2111-2118
Number of pages8
JournalMolecular Microbiology
Volume4
Issue number12
DOIs
StatePublished - Dec 1990
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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