Generation of halothane-induced immune response in a guinea pig model of halothane hepatitis

M. Siadat-Pajouh, A. K. Hubbard, T. P. Roth, A Jay Gandolfi

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

A guinea pig model of halothane hepatitis was used to explore the humoral immune response induced by multiple halothane exposures and the potential role this response might play in contributing to liver damage. Three different strains of guinea pigs (Strain 2, Amana, and Hartley) were exposed to 1% halothane under either 21 or 80% oxygen for 4 hr at 2-week intervals. In each strain, halothane induced the appearance of an antibody cross-reactive with trifluoroacetylated guinea pig serum albumin (TFA-GSA). Three of six Strain 2 guinea pigs demonstrated an association between antibody titer and serum glutamate pyruvate transaminase levels. However, the possible cause and effect relationship between these two factors requires more investigation. Hartley guinea pigs had a 4- to 11-fold higher level of anti-TFA antibody than the other two strains because of either a 'higher responder' genetic background or exposure conditions that favored oxidative metabolism of halothane. Immunization of Amana guinea pigs with TFA-GSA evoked a specific anti-TFA antibody response. However, the presence of this antibody before halothane exposure did not potentiate the transient liver damage induced by exposure. Thus, these results demonstrate that in guinea pigs multiple exposures to halothane induce the formation of an antibody that recognizes a reactive intermediate of halothane formed during the anesthetic's metabolism.

Original languageEnglish (US)
Pages (from-to)1209-1214
Number of pages6
JournalAnesthesia and Analgesia
Volume66
Issue number12
StatePublished - 1987

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Halothane
Guinea Pigs
Serum Albumin
Antibody Formation
Antibodies
Anti-Idiotypic Antibodies
Halothane Hepatitis
Liver
Humoral Immunity
Transaminases
Pyruvic Acid
Anesthetics
Glutamic Acid
Immunization
Oxygen
Serum

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine

Cite this

Generation of halothane-induced immune response in a guinea pig model of halothane hepatitis. / Siadat-Pajouh, M.; Hubbard, A. K.; Roth, T. P.; Gandolfi, A Jay.

In: Anesthesia and Analgesia, Vol. 66, No. 12, 1987, p. 1209-1214.

Research output: Contribution to journalArticle

Siadat-Pajouh, M. ; Hubbard, A. K. ; Roth, T. P. ; Gandolfi, A Jay. / Generation of halothane-induced immune response in a guinea pig model of halothane hepatitis. In: Anesthesia and Analgesia. 1987 ; Vol. 66, No. 12. pp. 1209-1214.
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