Generation of mice with a conditional allele for the transforming growth factor beta3 gene

Thomas C Doetschman, Teodora Georgieva, Hongqi Li, Thomas D. Reed, Christina Grisham, Jacqueline Friel, Mark A. Estabrook, Connie Gard, L. P. Sanford, Mohamad Azhar

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The transforming growth factor beta (TGFβ) pathway is involved in embryonic development and several inherited and acquired human diseases. The gene for TGFβ3 (Tgfb3) encodes one of the three ligands for TGFβ receptors. It is widely expressed in the embryo and its mutation or misexpression is found in human diseases. Tgfb3-/- mice die at birth from cleft palate, precluding functional studies in adults. Here, we generated mice in which exon 6 of Tgfb3 was flanked with LoxP sites (Tgfb3flox/flox). The adult mice were normal and fertile. EIIa-Cre-mediated deletion of exon 6 in Tgfb3flox/flox mice efficiently generated Tgfb3 conditional knockout (Tgfb3cko/cko) mice which died at birth from the same cleft palate defect as Tgfb3-/- mice, indicating that the conditional and knockout alleles are functionally equivalent. This Tgfb3cko allele will now enable studies of TGFβ3 function in different cell or tissue types in embryonic development and during adulthood.

Original languageEnglish (US)
Pages (from-to)59-66
Number of pages8
JournalGenesis
Volume50
Issue number1
DOIs
StatePublished - Jan 2012

Fingerprint

Transforming Growth Factor beta3
Alleles
Genes
Cleft Palate
Embryonic Development
Exons
Parturition
Transforming Growth Factor beta Receptors
Knockout Mice
Transforming Growth Factor beta
Embryonic Structures
Ligands
Mutation

Keywords

  • Autoimmunity
  • Cancer
  • Cardiovascular
  • Craniofacial
  • Neuromuscular
  • Transforming growth factor beta
  • Wound healing

ASJC Scopus subject areas

  • Endocrinology
  • Cell Biology
  • Genetics

Cite this

Doetschman, T. C., Georgieva, T., Li, H., Reed, T. D., Grisham, C., Friel, J., ... Azhar, M. (2012). Generation of mice with a conditional allele for the transforming growth factor beta3 gene. Genesis, 50(1), 59-66. https://doi.org/10.1002/dvg.20789

Generation of mice with a conditional allele for the transforming growth factor beta3 gene. / Doetschman, Thomas C; Georgieva, Teodora; Li, Hongqi; Reed, Thomas D.; Grisham, Christina; Friel, Jacqueline; Estabrook, Mark A.; Gard, Connie; Sanford, L. P.; Azhar, Mohamad.

In: Genesis, Vol. 50, No. 1, 01.2012, p. 59-66.

Research output: Contribution to journalArticle

Doetschman, TC, Georgieva, T, Li, H, Reed, TD, Grisham, C, Friel, J, Estabrook, MA, Gard, C, Sanford, LP & Azhar, M 2012, 'Generation of mice with a conditional allele for the transforming growth factor beta3 gene', Genesis, vol. 50, no. 1, pp. 59-66. https://doi.org/10.1002/dvg.20789
Doetschman, Thomas C ; Georgieva, Teodora ; Li, Hongqi ; Reed, Thomas D. ; Grisham, Christina ; Friel, Jacqueline ; Estabrook, Mark A. ; Gard, Connie ; Sanford, L. P. ; Azhar, Mohamad. / Generation of mice with a conditional allele for the transforming growth factor beta3 gene. In: Genesis. 2012 ; Vol. 50, No. 1. pp. 59-66.
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