Generation of mice with a conditional allele for transforming growth factor beta 1 gene

Mohamad Azhar, Moying Yin, Ramireddy Bommireddy, John J. Duffy, Junqi Yang, Sharon A. Pawlowski, Gregory P. Boivin, Sandra J. Engle, L. P. Sanford, Christina Grisham, Ram R. Singh, George F. Babcock, Thomas Doetschman

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

Transforming growth factor β1 (TGFβ1) is a multifunctional growth factor involved in wound healing, tissue fibrosis, and in the pathogenesis of many syndromic diseases (e.g., Marfan syndrome, Camurati-Engelmann disease) and muscular, neurological, ophthalmic, cardiovascular and immunological disorders, and cancer. Since the generation of Tgfb1 knockout mice, there has been extraordinary progress in understanding its physiological and pathophysiological function. Here, we report the generation of a conditional knockout allele for Tgfb1 in which its exon 6 is flanked with LoxP sites. As proof of principle, we crossed these mice to LckCre transgenic mice and specifically disrupted Tgfb1 in T cells. The results indicate that T-cell-produced TGFβ1 is required for normal in vivo regulation of peripheral T-cell activation, maintenance of T-cell homeostasis, and suppression of autoimmunity.

Original languageEnglish (US)
Pages (from-to)423-431
Number of pages9
JournalGenesis
Volume47
Issue number6
DOIs
StatePublished - Sep 22 2009

Keywords

  • Conditional knockout mice
  • Transforming growth factorβ

ASJC Scopus subject areas

  • Genetics
  • Endocrinology
  • Cell Biology

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    Azhar, M., Yin, M., Bommireddy, R., Duffy, J. J., Yang, J., Pawlowski, S. A., Boivin, G. P., Engle, S. J., Sanford, L. P., Grisham, C., Singh, R. R., Babcock, G. F., & Doetschman, T. (2009). Generation of mice with a conditional allele for transforming growth factor beta 1 gene. Genesis, 47(6), 423-431. https://doi.org/10.1002/dvg.20516