Genetic and structural analysis of G protein α subunit regulatory domains

G. L. Johnson, N. Dhanasekaran, S. K. Gupta, J. M. Lowndes, Richard Vaillancourt, A. E. Ruoho

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Genetic and structural analysis of the α chain polypeptides of heterotrimeric G proteins defines functional domains for GTP/GDP binding, GTPase activity, effector activation, receptor contact and βγ subunit complex regulation. The conservation in sequence comprising the GDP/GTP binding and GTPase domains among G protein α subunits readily allows common mutations to be made for the design of mutant polypeptides that function as constitutive active or dominant negative α chains when expressed in different cell types. Organization of the effector activation, receptor and βγ contact domains is similar in the primary sequence of the different α subunit polypeptides relative to the GTP/GDP binding domain sequences. Mutation with in common motifs of the different G protein α chain polypeptides have similar functional consequences. Thus, what has been learned with the Gs and Gi proteins and the regulation of adenylyl cyclase can be directly applied to the analysis of newly identified G proteins and their coupling to receptors and regulation of putative effector enzymes.

Original languageEnglish (US)
Pages (from-to)136-146
Number of pages11
JournalJournal of Cellular Biochemistry
Volume47
Issue number2
StatePublished - 1991
Externally publishedYes

Fingerprint

Protein Subunits
GTP-Binding Proteins
Structural analysis
Guanosine Triphosphate
Peptides
GTP Phosphohydrolases
Chemical activation
Heterotrimeric GTP-Binding Proteins
Mutation
Adenylyl Cyclases
Conservation
Enzymes
Proteins

Keywords

  • adenylyl cyclase
  • GDP
  • GTP
  • heterotrimeric G proteins
  • ion channels
  • phospholipases

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Cite this

Johnson, G. L., Dhanasekaran, N., Gupta, S. K., Lowndes, J. M., Vaillancourt, R., & Ruoho, A. E. (1991). Genetic and structural analysis of G protein α subunit regulatory domains. Journal of Cellular Biochemistry, 47(2), 136-146.

Genetic and structural analysis of G protein α subunit regulatory domains. / Johnson, G. L.; Dhanasekaran, N.; Gupta, S. K.; Lowndes, J. M.; Vaillancourt, Richard; Ruoho, A. E.

In: Journal of Cellular Biochemistry, Vol. 47, No. 2, 1991, p. 136-146.

Research output: Contribution to journalArticle

Johnson, GL, Dhanasekaran, N, Gupta, SK, Lowndes, JM, Vaillancourt, R & Ruoho, AE 1991, 'Genetic and structural analysis of G protein α subunit regulatory domains', Journal of Cellular Biochemistry, vol. 47, no. 2, pp. 136-146.
Johnson GL, Dhanasekaran N, Gupta SK, Lowndes JM, Vaillancourt R, Ruoho AE. Genetic and structural analysis of G protein α subunit regulatory domains. Journal of Cellular Biochemistry. 1991;47(2):136-146.
Johnson, G. L. ; Dhanasekaran, N. ; Gupta, S. K. ; Lowndes, J. M. ; Vaillancourt, Richard ; Ruoho, A. E. / Genetic and structural analysis of G protein α subunit regulatory domains. In: Journal of Cellular Biochemistry. 1991 ; Vol. 47, No. 2. pp. 136-146.
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