Genetic complementation of a glucocorticoid receptor deficiency by expression of cloned receptor cDNA

Roger Miesfeld, Sandro Rusconi, Paul J. Godowski, Bonnie A. Maler, Sam Okret, Ann Charlotte Wikström, Jan Åke Gustafsson, Keith R. Yamamoto

Research output: Contribution to journalArticlepeer-review

511 Scopus citations

Abstract

We isolated and sequenced 6.3 kb of cDNA encoding the rat glucocorticoid receptor, a protein that binds and activates a class of hormone-dependent transcriptional enhancers. Receptor-containing cells produce receptor mRNAs of ≅6.5 kb and ≅4.8 kb that differ only in their 3′ nontranslated regions; an open reading frame of 795 amino acids resides within the 5′ portion of the transcripts. The coding region was expressed in vitro, in transient transfections, and in stable transfectants of a receptor-deficient cell line. The protein products are indistinguishable from bona fide receptor with respect to sedimentation and electrophoretic mobility, antibody reactivity, and hormone and DNA binding. Moreover, the cloned receptor protein activates its corresponding enhancers, restoring to the receptor-deficient cells the full capacity for regulated enhancement.

Original languageEnglish (US)
Pages (from-to)389-399
Number of pages11
JournalCell
Volume46
Issue number3
DOIs
StatePublished - Aug 1 1986

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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