Genetic control of flocculation in Escherichia coli

Kimberly L. Ogden, Austin L. Taylor

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Escherichia coli cells form flocs or aggregates by overproducing type 1 pili. When the pil operon is placed under the control of a tac or lac promoter-operator sequence, the bacterial cells can be induced to form flocs by adding isopropyl-β-d-thiogalactopyranoside to the culture medium. This phenomenon of genetically induced flocculation can aid in the downstream processing of biological products. This paper describes the construction of two artificially controlled plasmids which cause cell flocculation. Cell aggregates 50 μm in mean diameter were obtained 1 h after the cells were induced.

Original languageEnglish (US)
Pages (from-to)279-286
Number of pages8
JournalJournal of Industrial Microbiology
Volume7
Issue number4
DOIs
StatePublished - Jun 1 1991
Externally publishedYes

Keywords

  • Bacterial aggregation
  • Induction
  • Type 1 pili

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

Fingerprint Dive into the research topics of 'Genetic control of flocculation in Escherichia coli'. Together they form a unique fingerprint.

  • Cite this