Genetic evidence that ZFY is not the testis-determining factor

M. S. Palmer, A. H. Sinclair, P. Berta, Nathan Ellis, P. N. Goodfellow, N. E. Abbas, M. Fellous

Research output: Contribution to journalArticle

228 Citations (Scopus)

Abstract

IN mammals, the testis determining gene (TDF), present on the Y chromosome, induces the undifferentiated gonads to form testes1. The position of TDF on the human Y chromosome has been defined by analysing the genomes of XX males and XY females2, generated by abnormal genetic exchange between the X and Y chromosomes in male meiosis3. In this way TDF has been localized close to the pseudoautosomal region shared by the sex chromosomes, in the distal Y-specific region. A recently cloned human gene, ZFY, has many features indicating that it is TDF4-6. For example, ZFY encodes a protein with many features of a transcription factor including a domain with multiple 'zinc-finger' motifs4-6. Less consistent with ZFY being TDF, however, is the presence of a very similar gene, ZFX, on the X chromosome4,7, and the presence of a sequence related to ZFY on autosomes in marsupials8. We now report on analysis of XX males lacking ZFY. In these individuals, the male phenotype could be explained by a mutation in a gene 'downstream' of ZFY in the sex-determining hierarchy; but in that case there should be no exchange of material between the X and Y chromosomes. We find on the contrary that in 4 XX males lacking ZFY, there is exchange of Y-specific sequences next to the pseudoautosomal boundary, redefining the region in which TDF must lie.

Original languageEnglish (US)
Pages (from-to)937-939
Number of pages3
JournalNature
Volume342
Issue number6252
StatePublished - 1989
Externally publishedYes

Fingerprint

Sex-Determining Region Y Protein
Y Chromosome
X Chromosome
Genes
Chromosomes, Human, Y
Sex Chromosomes
Zinc Fingers
Gonads
Testis
Mammals
Transcription Factors
Genome
Phenotype
Mutation

ASJC Scopus subject areas

  • General

Cite this

Palmer, M. S., Sinclair, A. H., Berta, P., Ellis, N., Goodfellow, P. N., Abbas, N. E., & Fellous, M. (1989). Genetic evidence that ZFY is not the testis-determining factor. Nature, 342(6252), 937-939.

Genetic evidence that ZFY is not the testis-determining factor. / Palmer, M. S.; Sinclair, A. H.; Berta, P.; Ellis, Nathan; Goodfellow, P. N.; Abbas, N. E.; Fellous, M.

In: Nature, Vol. 342, No. 6252, 1989, p. 937-939.

Research output: Contribution to journalArticle

Palmer, MS, Sinclair, AH, Berta, P, Ellis, N, Goodfellow, PN, Abbas, NE & Fellous, M 1989, 'Genetic evidence that ZFY is not the testis-determining factor', Nature, vol. 342, no. 6252, pp. 937-939.
Palmer MS, Sinclair AH, Berta P, Ellis N, Goodfellow PN, Abbas NE et al. Genetic evidence that ZFY is not the testis-determining factor. Nature. 1989;342(6252):937-939.
Palmer, M. S. ; Sinclair, A. H. ; Berta, P. ; Ellis, Nathan ; Goodfellow, P. N. ; Abbas, N. E. ; Fellous, M. / Genetic evidence that ZFY is not the testis-determining factor. In: Nature. 1989 ; Vol. 342, No. 6252. pp. 937-939.
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