Genetic mapping of resistance to Bacillus thuringiensis toxins in diamondback moth using biphasic linkage analysis

David G. Heckel, Linda J. Gahan, Yong Biao Liu, Bruce E Tabashnik

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

Transgenic plants producing environmentally benign Bacillus thuringiensis (Bt) toxins are deployed increasingly for insect control, but their efficacy will be short-lived if pests adapt quickly. The diamondback moth (Plutella xylostella), a worldwide pest of vegetables, is the first insect to evolve resistance to Bt toxins in open-field populations. A recessive autosomal gene confers resistance to at least four Bt toxins and enables survival without adverse effects on transgenic plants. Allelic variants of this gene confer resistance in strains from Hawaii, Pennsylvania, and the Philippines. Here we exploited the biphasic nature of Lepidopteran genetic linkage to map this gene in diamondback moth with 207 amplified fragment length polymorphisms as DNA markers. We also cloned and sequenced an amplified fragment length polymorphism marker for the chromosome containing the Bt resistance gene. The results provide a powerful tool for facilitating progress in understanding, monitoring, and managing resistance to Bt.

Original languageEnglish (US)
Pages (from-to)8373-8377
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume96
Issue number15
DOIs
StatePublished - Jul 20 1999

Fingerprint

Bacillus thuringiensis
Moths
Genetically Modified Plants
Genetic Markers
Insect Control
Genes
Recessive Genes
Genetic Linkage
Philippines
Vegetables
Insects
Population

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Genetic mapping of resistance to Bacillus thuringiensis toxins in diamondback moth using biphasic linkage analysis. / Heckel, David G.; Gahan, Linda J.; Liu, Yong Biao; Tabashnik, Bruce E.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, No. 15, 20.07.1999, p. 8373-8377.

Research output: Contribution to journalArticle

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