Genetic mapping of the barley nitrate reductase-deficient nar1 and nar2 loci

J. M. Melzer, A. Kleinhofs, David A Kudrna, R. L. Warner, T. K. Blake

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The nar2 locus that codes for a protein involved in molybdenum cofactor function in nitrate reductase and other molybdoenzymes was mapped to barley chromosome 7. F2 genotypic data from F3 head rows indicated nar2 is located 8.4±2.1 and 23.0± 4.6 cm from the narrow leaf dwarf (nld) and mottled seedling (mt2) loci, respectively. This locates the nar2 locus at 54.7±3.1 cm from the short-haired rachilla (s) locus near the centromere of chromosome 7. Close linkage of nar2 with DDT resistance (ddt) and high lysine (lys3) loci was detected but could not be quantified due to deviations from the individual expected 1:2:1 segregations for the ddt and lys3 genes. Southern blots of wheat-barley addition lines probed with a nitrate reductase cDNA located the NADH : nitrate reductase structural gene, nar1, to chromosome 6.

Original languageEnglish (US)
Pages (from-to)767-771
Number of pages5
JournalTheoretical And Applied Genetics
Volume75
Issue number5
DOIs
StatePublished - May 1988
Externally publishedYes

Fingerprint

Nitrate Reductase
Chromosomes, Human, Pair 7
Hordeum
nitrate reductase
chromosome mapping
Nitrate Reductase (NADH)
barley
Chromosomes, Human, Pair 6
loci
DDT
Centromere
Southern Blotting
Seedlings
Genes
Lysine
Triticum
chromosomes
Complementary DNA
Head
nitrate reductase (NADH)

Keywords

  • Hordeum vulgare
  • Linkage
  • Mutants
  • Nitrate reductase

ASJC Scopus subject areas

  • Genetics(clinical)
  • Genetics
  • Plant Science
  • Horticulture
  • Agronomy and Crop Science

Cite this

Genetic mapping of the barley nitrate reductase-deficient nar1 and nar2 loci. / Melzer, J. M.; Kleinhofs, A.; Kudrna, David A; Warner, R. L.; Blake, T. K.

In: Theoretical And Applied Genetics, Vol. 75, No. 5, 05.1988, p. 767-771.

Research output: Contribution to journalArticle

Melzer, J. M. ; Kleinhofs, A. ; Kudrna, David A ; Warner, R. L. ; Blake, T. K. / Genetic mapping of the barley nitrate reductase-deficient nar1 and nar2 loci. In: Theoretical And Applied Genetics. 1988 ; Vol. 75, No. 5. pp. 767-771.
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