Genetic variation and mtCOI phylogeny for Bemisia tabaci (Hemiptera, Aleyrodidae) indicate that the 'B' biotype predominates in Iran

Shoorcheh H. Rajaei, B. Kazemi, S. Manzari, Judith K Brown, A. Sarafrazi

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Despite a large number of investigations on the molecular genetics and population structure of the whitefly Bemisia tabaci (Gennadius) complex, no such study had been conducted in Iran. The genetic variation of B. tabaci was examined using polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) for 18 field collections from cucumber, eggplant, and tomato in four provinces of Iran. PCR amplification and restriction digestion with two enzymes detected 388 RFLP fragments, of which 16 fragments showed polymorphisms. Cluster analysis of these data placed all B. tabaci individuals within a single group, and there was no evidence for between- or within-population genetic variation. Phylogenetic (Clustal W) analysis of 42 B. tabaci mtCOI sequences (n = 21 field collections) from Iran, and a comparison with well-studied haplotype or biotype reference sequences available in public sequence databases, revealed that the Iranian B. tabaci populations were most closely related to the B biotype at 0-1.2% nucleotide identity. The B biotype is a well-known member of a sister clade from the Middle East-North African region of the world, owing to its nearly worldwide distribution and invasive characteristics. This report indicates that a single major haplotype of B biotype is prevalent in Iran and that its closest relative is the B biotype. Also, given the extent of known variation in the Middle East and African continent, data indicate somewhat surprisingly that the B. tabaci collections sampled in Iran had limited genetic variation and population substructure. Knowledge that the B biotype of B. tabaci predominates in Iran is important for designing effective pest management strategies given that biotypes of B. tabaci are known to differ greatly with respect to insecticide resistance, host range, virus-vector interactions, and other key biological characteristics.

Original languageEnglish (US)
Pages (from-to)199-206
Number of pages8
JournalJournal of Pest Science
Volume81
Issue number4
DOIs
StatePublished - 2008

Fingerprint

Bemisia tabaci
Aleyrodidae
biotypes
Hemiptera
Iran
genetic variation
phylogeny
Middle East
haplotypes
restriction fragment length polymorphism
Bemisia argentifolii
insecticide resistance
eggplants
pest management
host range
molecular genetics
cucumbers
population genetics
cluster analysis
population structure

Keywords

  • Bemisia tabaci
  • Genetic variation
  • Iran
  • mtCOI
  • PCR-RFLP
  • Phylogeny

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

Genetic variation and mtCOI phylogeny for Bemisia tabaci (Hemiptera, Aleyrodidae) indicate that the 'B' biotype predominates in Iran. / Rajaei, Shoorcheh H.; Kazemi, B.; Manzari, S.; Brown, Judith K; Sarafrazi, A.

In: Journal of Pest Science, Vol. 81, No. 4, 2008, p. 199-206.

Research output: Contribution to journalArticle

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abstract = "Despite a large number of investigations on the molecular genetics and population structure of the whitefly Bemisia tabaci (Gennadius) complex, no such study had been conducted in Iran. The genetic variation of B. tabaci was examined using polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) for 18 field collections from cucumber, eggplant, and tomato in four provinces of Iran. PCR amplification and restriction digestion with two enzymes detected 388 RFLP fragments, of which 16 fragments showed polymorphisms. Cluster analysis of these data placed all B. tabaci individuals within a single group, and there was no evidence for between- or within-population genetic variation. Phylogenetic (Clustal W) analysis of 42 B. tabaci mtCOI sequences (n = 21 field collections) from Iran, and a comparison with well-studied haplotype or biotype reference sequences available in public sequence databases, revealed that the Iranian B. tabaci populations were most closely related to the B biotype at 0-1.2{\%} nucleotide identity. The B biotype is a well-known member of a sister clade from the Middle East-North African region of the world, owing to its nearly worldwide distribution and invasive characteristics. This report indicates that a single major haplotype of B biotype is prevalent in Iran and that its closest relative is the B biotype. Also, given the extent of known variation in the Middle East and African continent, data indicate somewhat surprisingly that the B. tabaci collections sampled in Iran had limited genetic variation and population substructure. Knowledge that the B biotype of B. tabaci predominates in Iran is important for designing effective pest management strategies given that biotypes of B. tabaci are known to differ greatly with respect to insecticide resistance, host range, virus-vector interactions, and other key biological characteristics.",
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