Genetic variation of white spot syndrome virus (WSSV) in Pacific white shrimp Litopenaeus vannamei (Boone 1931) culture of Thailand

Patharapol Piamsomboon, Tidaporn Chaweepack, Jee Eun Han, Feng-Jyu Tang-Nelson, Janenuj Wongtavatchai

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

White spot syndrome virus (WSSV) is highly pathogenic to penaeid shrimp and has caused significant economic losses in the shrimp farming industry in Thailand. Genotyping analysis was done in 124 WSSV isolates from cultured Pacific white shrimp Litopenaeus vannamei. These samples were obtained during 2007–2014 from eight provinces in Thailand. We investigated five variable loci in the virus genome: deletions in two variable regions, VR14/15 and VR23/24, and three variable number tandem repeats (VNTR) located in open reading frame (ORF) 75, 125 and 94. WSSV genotype was characterized as (X14/15, X23/24) (N75-N125-N94) where X is the number of base pair deletion in the variable region and N is the number of repeat units (RUs) in a specific ORF. The deletion pattern in VR14/15 and VR23/24 regions characterized three WSSV genotypes. The most prevalent genotype was (595014/15, 1097123/24), and it was found in all studied areas. At least 33 genotypes of WSSV were analysed based on 3 VNTR loci, indicating that the VNTRs of WSSV genome are highly variable. From 124 WSSV samples, two samples presented the characteristic of all five variable loci similar to WSSV collected during 2010 in Saudi Arabia (595014/15, 1097123/24) (375-6125-794). Many different WSSV genotypes shown in this study as compared to previously reported genotypes in Thailand suggests current status of disease epidemiology, as well as probable movements of WSSV between countries.

Original languageEnglish (US)
Pages (from-to)265-272
Number of pages8
JournalAquaculture Research
Volume49
Issue number1
DOIs
StatePublished - Jan 1 2018
Externally publishedYes

Fingerprint

White spot syndrome virus
Litopenaeus vannamei
Thailand
genetic variation
virus
shrimp
genotype
minisatellite repeats
open reading frames
genome
loci
Penaeidae
Saudi Arabia
sampling
epidemiology
genotyping
farming systems
industry
economics
viruses

Keywords

  • Litopenaeus vannamei
  • variable loci
  • virus genotyping
  • white spot disease

ASJC Scopus subject areas

  • Aquatic Science

Cite this

Genetic variation of white spot syndrome virus (WSSV) in Pacific white shrimp Litopenaeus vannamei (Boone 1931) culture of Thailand. / Piamsomboon, Patharapol; Chaweepack, Tidaporn; Han, Jee Eun; Tang-Nelson, Feng-Jyu; Wongtavatchai, Janenuj.

In: Aquaculture Research, Vol. 49, No. 1, 01.01.2018, p. 265-272.

Research output: Contribution to journalArticle

Piamsomboon, Patharapol ; Chaweepack, Tidaporn ; Han, Jee Eun ; Tang-Nelson, Feng-Jyu ; Wongtavatchai, Janenuj. / Genetic variation of white spot syndrome virus (WSSV) in Pacific white shrimp Litopenaeus vannamei (Boone 1931) culture of Thailand. In: Aquaculture Research. 2018 ; Vol. 49, No. 1. pp. 265-272.
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