Genome-wide analysis identifies a general requirement for polarity proteins in endocytic traffic

Zita Balklava, Saumya Pant, Hanna F Fares, Barth D. Grant

Research output: Contribution to journalArticle

197 Citations (Scopus)

Abstract

In a genome-wide RNA-mediated interference screen for genes required in membrane traffic - including endocytic uptake, recycling from endosomes to the plasma membrane, and secretion - we identified 168 candidate endocytosis regulators and 100 candidate secretion regulators. Many of these candidates are highly conserved among metazoans but have not been previously implicated in these processes. Among the positives from the screen, we identified PAR-3, PAR-6, PKC-3 and CDC-42, proteins that are well known for their importance in the generation of embryonic and epithelial-cell polarity. Further analysis showed that endocytic transport in Caenorhabditis elegans coelomocytes and human HeLa cells was also compromised after perturbation of CDC-42/Cdc42 or PAR-6/Par6 function, indicating a general requirement for these proteins in regulating endocytic traffic. Consistent with these results, we found that tagged CDC-42/Cdc42 is enriched on recycling endosomes in C. elegans and mammalian cells, suggesting a direct function in the regulation of transport.

Original languageEnglish (US)
Pages (from-to)1066-1073
Number of pages8
JournalNature Cell Biology
Volume9
Issue number9
DOIs
StatePublished - Sep 2007

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Centers for Disease Control and Prevention (U.S.)
Endosomes
Caenorhabditis elegans
Genome
Cell Polarity
Proteins
Recycling
RNA Interference
Endocytosis
HeLa Cells
Epithelial Cells
Cell Membrane
Membranes
Genes

ASJC Scopus subject areas

  • Cell Biology

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Genome-wide analysis identifies a general requirement for polarity proteins in endocytic traffic. / Balklava, Zita; Pant, Saumya; Fares, Hanna F; Grant, Barth D.

In: Nature Cell Biology, Vol. 9, No. 9, 09.2007, p. 1066-1073.

Research output: Contribution to journalArticle

Balklava, Zita ; Pant, Saumya ; Fares, Hanna F ; Grant, Barth D. / Genome-wide analysis identifies a general requirement for polarity proteins in endocytic traffic. In: Nature Cell Biology. 2007 ; Vol. 9, No. 9. pp. 1066-1073.
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