Genome-wide association study provides evidence for a breast cancer risk locus at 6q22.33

Bert Gold, Tomas Kirchhoff, Stefan Stefanov, James Lautenberger, Agnes Viale, Judy Garber, Eitan Friedman, Steven Narod, Adam B. Olshen, Peter Gregersen, Kristi Kosarin, Adam Olsh, Julie Bergeron, Nathan A. Ellis, Robert J. Klein, Andrew G. Clark, Larry Norton, Michael Dean, Jeff Boyd, Kenneth Offit

Research output: Contribution to journalArticle

255 Scopus citations

Abstract

We performed a three-phase genome-wide association study (GWAS) using cases and controls from a genetically isolated population, Ashkenazi Jews (AJ), to identify loci associated with breast cancer risk. In the first phase, we compared allele frequencies of 150,080 SNPs in 249 high-risk, BRCA1/2 mutation-negative AJ familial cases and 299 cancer-free AJ controls using χ2 and the Cochran-Armitage trend tests. In the second phase, we genotyped 343 SNPs from 123 regions most significantly associated from stage 1, including 4 SNPs from the FGFR2 region, in 950 consecutive AJ breast cancer cases and 979 age-matched AJ controls. We replicated major associations in a third independent set of 243 AJ cases and 187 controls. We obtained a significant allele P value of association with AJ breast cancer in the FGFR2 region (P = 1.5 × 10-5, odds ratio (OR) 1.26, 95% confidence interval (CI) 1.13-1.40 at rs1078806 for all phases combined). In addition, we found a risk locus in a region of chromosome 6q22.33 (P = 2.9 × 10 -8, OR 1.41, 95% CI 1.25-1.59 at rs2180341). Using several SNPs at each implicated locus, we were able to verify associations and impute haplotypes. The major haplotype at the 6q22.33 locus conferred protection from disease, whereas the minor haplotype conferred risk. Candidate genes in the 6q22.33 region include ECHDC1, which encodes a protein involved in mitochondrial fatty acid oxidation, and also RNF146, which encodes a ubiquitin protein ligase, both known pathways in breast cancer pathogenesis.

Original languageEnglish (US)
Pages (from-to)4340-4345
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume105
Issue number11
DOIs
StatePublished - Mar 18 2008
Externally publishedYes

Keywords

  • Disease
  • Genomics
  • Mapping
  • Predisposition
  • SNP

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Genome-wide association study provides evidence for a breast cancer risk locus at 6q22.33'. Together they form a unique fingerprint.

  • Cite this

    Gold, B., Kirchhoff, T., Stefanov, S., Lautenberger, J., Viale, A., Garber, J., Friedman, E., Narod, S., Olshen, A. B., Gregersen, P., Kosarin, K., Olsh, A., Bergeron, J., Ellis, N. A., Klein, R. J., Clark, A. G., Norton, L., Dean, M., Boyd, J., & Offit, K. (2008). Genome-wide association study provides evidence for a breast cancer risk locus at 6q22.33. Proceedings of the National Academy of Sciences of the United States of America, 105(11), 4340-4345. https://doi.org/10.1073/pnas.0800441105