Genome-wide CRISPR screen reveals autophagy disruption as the convergence mechanism that regulates the Nrf2 transcription factor

Michael J. Kerins, Pengfei Liu, Wang Tian, William Mannheim, Donna Zhang, Aikseng Ooi

Research output: Contribution to journalArticle

Abstract

The nuclear factor (erythroid 2)-like 2 (NRF2 or NFE2L2) transcription factor regulates the expression of many genes that are critical in maintaining cellular homeostasis. Its deregulation has been implicated in many diseases, including cancer and metabolic and neurodegenerative diseases. While several mechanisms by which NRF2 can be activated have gradually been identified over time, a more complete regulatory network of NRF2 is still lacking. Here we show through a genome-wide clustered regularly interspaced short palindromic repeat (CRISPR) screen that a total of 273 genes, when knocked out, will lead to sustained NRF2 activation. Pathway analysis revealed a significant overrepresentation of genes (18 of the 273 genes) involved in autophagy. Molecular validation of a subset of the enriched genes identified 8 high-confidence genes that negatively regulate NRF2 activity irrespective of cell type: ATG12, ATG7, GOSR1, IFT172, NRXN2, RAB6A, VPS37A, and the well-known negative regulator of NRF2, KEAP1. Of these, ATG12, ATG7, KEAP1, and VPS37A are known to be involved in autophagic processes. Our results present a comprehensive list of NRF2 negative regulators and reveal an intimate link between autophagy and NRF2 regulation.

Original languageEnglish (US)
Article number00037-19
JournalMolecular and cellular biology
Volume39
Issue number13
DOIs
StatePublished - Jan 1 2019

Fingerprint

Clustered Regularly Interspaced Short Palindromic Repeats
Autophagy
Transcription Factors
Genome
Genes
Metabolic Diseases
Neurodegenerative Diseases
Homeostasis
Gene Expression
Neoplasms

Keywords

  • Autophagy
  • CRISPR
  • Gene reporters
  • Genomics
  • KEAP1
  • Molecular biology
  • NFE2L2
  • NRF2
  • Oxidative stress
  • Signal transduction

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Genome-wide CRISPR screen reveals autophagy disruption as the convergence mechanism that regulates the Nrf2 transcription factor. / Kerins, Michael J.; Liu, Pengfei; Tian, Wang; Mannheim, William; Zhang, Donna; Ooi, Aikseng.

In: Molecular and cellular biology, Vol. 39, No. 13, 00037-19, 01.01.2019.

Research output: Contribution to journalArticle

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