Genomic and phenotypic response of hornyhead turbot exposed to municipal wastewater effluents

Doris E. Vidal-Dorsch, Steven M. Bay, Cataldo Ribecco, L. James Sprague, Mila Angert, Colleen Ludka, Eugenia Ricciardelli, Oliana Carnevali, Darrin J. Greenstein, Daniel Schlenk, Kevin M. Kelley, Jesus A. Reyes, Shane A Snyder, Brett Vanderford, Lan C. Wiborg, Dawn Petschauer, Roman Sasik, Michael Baker, Gary Hardiman

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Laboratory tests with marine flatfish were conducted to investigate associations among gene expression, higher biological responses and wastewater effluent exposure. In the present study, male hornyhead turbot (Pleuronichthys verticalis) were exposed to environmentally realistic (0.5%) and higher (5%) concentrations of chemically enhanced advanced-primary (PL) and full-secondary treated (HTP) effluents from two southern California wastewater treatment plants (WWTP). Hepatic gene expression was examined using a custom low-density microarray. Alterations in gene expression (vs. controls) were observed in fish exposed to both effluent types. Fish exposed to 0.5% PL effluent showed changes in genes involved in the metabolism of xenobiotics, steroids, and lipids, among other processes. Fish exposed to 5% PL effluent showed expression changes in genes involved in carbohydrate metabolism, stress responses, xenobiotic metabolism, and steroid synthesis, among others. Exposure to 5% HTP effluent changed the expression of genes involved in lipid, glutathione and xenobiotic metabolism, as well as immune responses. Although no concentration-dependent patterns of response to effluent exposure were found, significant Spearman correlations were observed between the expression of 22 genes and molecular and/or higher biological responses. These results indicate that microarray gene expression data correspond to higher biological responses and should be incorporated in studies assessing fish health after exposure to complex environmental mixtures.

Original languageEnglish (US)
Pages (from-to)174-184
Number of pages11
JournalAquatic Toxicology
Volume140-141
DOIs
Publication statusPublished - Sep 5 2013
Externally publishedYes

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Keywords

  • Effluent
  • Gene expression
  • Marine flatfish

ASJC Scopus subject areas

  • Aquatic Science
  • Health, Toxicology and Mutagenesis

Cite this

Vidal-Dorsch, D. E., Bay, S. M., Ribecco, C., Sprague, L. J., Angert, M., Ludka, C., ... Hardiman, G. (2013). Genomic and phenotypic response of hornyhead turbot exposed to municipal wastewater effluents. Aquatic Toxicology, 140-141, 174-184. https://doi.org/10.1016/j.aquatox.2013.05.017