Genomic profiles of damage and protection in human intracerebral hemorrhage

S. Thomas Carmichael, Paul M. Vespa, Jeffery L. Saver, Giovanni Coppola, Daniel H. Geschwind, Sidney Starkman, Chad M. Miller, Stella Kidwell, David S. Liebeskind, Neil A. Martin

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Intracerebral hemorrhage (ICH) produces a high rate of death and disability. The molecular mechanisms of damage in perihematomal tissue in humans have not been systematically characterized. This study determines the gene expression profile and molecular networks that are induced in human perihematomal tissue through molecular analysis of tissue obtained from endoscopic clot evacuation. Differentially expressed genes and their cellular origin were confirmed in a mouse model of ICH. A total of 624 genes showed altered regulation in human ICH. Bioinformatic analysis shows that these genes form interconnected networks of proinflammatory, anti-inflammatory, and neuronal signaling cascades. Intracerebral hemorrhage evokes coordinated upregulation of proinflammatory signaling through specific cytokines and chemokines and their downstream molecular pathways. Anti-inflammatory networks are also induced by ICH, including annexins A1 and A2 and transforming growth factor beta (TGFβ) and their intracellular cascades. Intracerebral hemorrhage downregulates many neuronal signaling systems, including the N-methyl-D-aspartic acid (NMDA) receptor complex and membrane ion channels. Select portions of these molecular networks were confirmed in the mouse, and the proteins in a subset of these networks localized to subsets of neurons, oligodendrocytes, or leukocytes. These inflammatory and anti-inflammatory networks interact at several key points in neutrophil signaling, apoptotic cell death, and protease responses, and indicate that secondary damage in ICH activates opposing molecular systems.

Original languageEnglish (US)
Pages (from-to)1860-1875
Number of pages16
JournalJournal of Cerebral Blood Flow and Metabolism
Volume28
Issue number11
DOIs
StatePublished - Nov 2008
Externally publishedYes

Fingerprint

Cerebral Hemorrhage
Anti-Inflammatory Agents
Ion Channels
Annexin A2
Annexin A1
Genes
Oligodendroglia
N-Methylaspartate
Computational Biology
Transcriptome
Chemokines
Transforming Growth Factor beta
Neutrophils
Leukocytes
Peptide Hydrolases
Cell Death
Up-Regulation
Down-Regulation
Cytokines
Neurons

Keywords

  • Annexin
  • Anti-inflammatory
  • Chemokine
  • Microarray
  • Neuroprotection
  • Perihematomal

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Clinical Neurology
  • Neurology

Cite this

Carmichael, S. T., Vespa, P. M., Saver, J. L., Coppola, G., Geschwind, D. H., Starkman, S., ... Martin, N. A. (2008). Genomic profiles of damage and protection in human intracerebral hemorrhage. Journal of Cerebral Blood Flow and Metabolism, 28(11), 1860-1875. https://doi.org/10.1038/jcbfm.2008.77

Genomic profiles of damage and protection in human intracerebral hemorrhage. / Carmichael, S. Thomas; Vespa, Paul M.; Saver, Jeffery L.; Coppola, Giovanni; Geschwind, Daniel H.; Starkman, Sidney; Miller, Chad M.; Kidwell, Stella; Liebeskind, David S.; Martin, Neil A.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 28, No. 11, 11.2008, p. 1860-1875.

Research output: Contribution to journalArticle

Carmichael, ST, Vespa, PM, Saver, JL, Coppola, G, Geschwind, DH, Starkman, S, Miller, CM, Kidwell, S, Liebeskind, DS & Martin, NA 2008, 'Genomic profiles of damage and protection in human intracerebral hemorrhage', Journal of Cerebral Blood Flow and Metabolism, vol. 28, no. 11, pp. 1860-1875. https://doi.org/10.1038/jcbfm.2008.77
Carmichael ST, Vespa PM, Saver JL, Coppola G, Geschwind DH, Starkman S et al. Genomic profiles of damage and protection in human intracerebral hemorrhage. Journal of Cerebral Blood Flow and Metabolism. 2008 Nov;28(11):1860-1875. https://doi.org/10.1038/jcbfm.2008.77
Carmichael, S. Thomas ; Vespa, Paul M. ; Saver, Jeffery L. ; Coppola, Giovanni ; Geschwind, Daniel H. ; Starkman, Sidney ; Miller, Chad M. ; Kidwell, Stella ; Liebeskind, David S. ; Martin, Neil A. / Genomic profiles of damage and protection in human intracerebral hemorrhage. In: Journal of Cerebral Blood Flow and Metabolism. 2008 ; Vol. 28, No. 11. pp. 1860-1875.
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