Genomic resources for gene discovery, functional genome annotation, and evolutionary studies of maize and its close relatives

Chao Wang, Xue Shi, Lin Liu, Haiyan Li, Jetty S.S. Ammiraju, David A. Kudrna, Wentao Xiong, Hao Wang, Zhaozhao Dai, Yonglian Zheng, Jinsheng Lai, Weiwei Jin, Joachim Messing, Jeffrey L. Bennetzen, Rod A. Wing, Meizhong Luo

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Maize is one of the most important food crops and a key model for genetics and developmental biology. A genetically anchored and high-quality draft genome sequence of maize inbred B73 has been obtained to serve as a reference sequence. To facilitate evolutionary studies in maize and its close relatives, much like the Oryza Map Alignment Project (OMAP) (www.OMAP.org) bacterial artificial chromosome (BAC) resource did for the rice community, we constructed BAC libraries for maize inbred lines Zheng58, Chang7-2, and Mo17 and maize wild relatives Zea mays ssp. parviglumis and Tripsacum dactyloides. Furthermore, to extend functional genomic studies to maize and sorghum, we also constructed binary BAC (BIBAC) libraries for the maize inbred B73 and the sorghum landrace Nengsi-1. The BAC/BIBAC vectors facilitate transfer of large intact DNA inserts from BAC clones to the BIBAC vector and functional complementation of large DNA fragments. These seven Zea Map Alignment Project (ZMAP) BAC/BIBAC libraries have average insert sizes ranging from 92 to 148 kb, organellar DNA from 0.17 to 2.3%, empty vector rates between 0.35 and 5.56%, and genome equivalents of 4.7-to 8.4-fold. The usefulness of the Parviglumis and Tripsacum BAC libraries was demonstrated by mapping clones to the reference genome. Novel genes and alleles present in these ZMAP libraries can now be used for functional complementation studies and positional or homology-based cloning of genes for translational genomics.

Original languageEnglish (US)
Pages (from-to)723-737
Number of pages15
JournalGenetics
Volume195
Issue number3
DOIs
StatePublished - Nov 11 2013

ASJC Scopus subject areas

  • Genetics

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