Genomic sequencing and analysis of Chilli ringspot virus, a novel potyvirus

Dian Gong, Jian Hua Wang, Zhan Song Lin, Shao Yan Zhang, Yu Liang Zhang, Nai Tong Yu, Zhongguo Xiong, Zhi Xin Liu

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Chilli ringspot virus (ChiRSV), a novel potyvirus, was recently found in Hainan, China with high prevalence. The genomic sequence of the ChiRSV-HN/14 isolate was determined by sequencing overlapping cDNA segments generated by reverse transcription polymerase chain reaction with degenerate and/or specific primers. ChiRSV genome (GenBank Acc. no. JN008909) comprised of 9,571 nucleotides (nt) excluding the 30-terminal poly (A) tail and contained a large open reading frame of 9,240 nt encoding a large polyprotein of 3,079 amino acids with predicted Mr of 349.1 kDa. A small, overlapping PIPO coding region was also found to span from nt 2,913 to 3,095, with a capacity to encode a peptide of 60 amino acids. ChiRSV shares sequence identities of only 48.5-65.4 and 42.9-68.7% with closely related potyviruses at the nucleotide and the amino acid levels, respectively. Phylogenetic analysis of the genomic sequences provided further evidence that ChiRSV is a distinct species of the Potyvirus genus. ChiRSV-HN/14 is most closely related to Tobacco vein banding mosaic virus and two other pepper-infecting potyviruses.

Original languageEnglish (US)
Pages (from-to)439-444
Number of pages6
JournalVirus Genes
Volume43
Issue number3
DOIs
StatePublished - Dec 1 2011

Keywords

  • ChiRSV
  • Genome
  • Motif
  • Phylogeny
  • Potyvirus

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Virology

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    Gong, D., Wang, J. H., Lin, Z. S., Zhang, S. Y., Zhang, Y. L., Yu, N. T., Xiong, Z., & Liu, Z. X. (2011). Genomic sequencing and analysis of Chilli ringspot virus, a novel potyvirus. Virus Genes, 43(3), 439-444. https://doi.org/10.1007/s11262-011-0652-2