Germ cell-specific expression of a gene encoding eukaryotic translation elongation factor 1α (eEF-1α) and generation of eEF-1α; retropseudogenes in Xenopus laevis

Bassima Abdallah, Jacques Hourdry, Paul A Krieg, Herman Denis, André Mazabraud

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

We have studied by in situ hybridization the expression of the genes encoding the somatic form and the oocyte form of Xenopus laevis eEF-1α. The somatic form of eEF-1α (eEF-1αS) mRNA is virtually undetectable in male and female germ cells of the adult gonad but is very abundant in embryonic cells after the neurula stage. In contrast, another form of eEF-1α (eEF-1αO) mRNA is highly concentrated in oogonia and in previtellogenic oocytes but is undetectable in eggs and embryos. eEF-1αO mRNA is also present in spermatogonia and spermatocytes of adult testis. The latter finding identifies eEF-1αO mRNA as a germ cell-specific gene product. Although germ cells contain very little eEF-1αS mRNA, several eEF-1αS retropseudogenes exist in X. laevis chromosomes. These genes are thought to arise in germ cells from reverse transcription of mRNA and subsequent integration of the cDNA copies into chromosomal DNA. We suggest that eEF-1αS pseudogenes are generated in primordial germ cells of the embryo before they differentiate into oogonia or spermatogonia.

Original languageEnglish (US)
Pages (from-to)9277-9281
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume88
Issue number20
StatePublished - Oct 15 1991
Externally publishedYes

Fingerprint

Peptide Elongation Factor 1
Xenopus laevis
Germ Cells
Gene Expression
Messenger RNA
Oogonia
Spermatogonia
Oocytes
Embryonic Structures
Pseudogenes
Spermatocytes
Gonads
X Chromosome
Eggs
Genes
Reverse Transcription
In Situ Hybridization
Testis
Complementary DNA
DNA

Keywords

  • Oocyte form of eukaryotic translation elongation factor 1α
  • Processed pseudogenes

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Germ cell-specific expression of a gene encoding eukaryotic translation elongation factor 1α (eEF-1α) and generation of eEF-1α; retropseudogenes in Xenopus laevis. / Abdallah, Bassima; Hourdry, Jacques; Krieg, Paul A; Denis, Herman; Mazabraud, André.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, No. 20, 15.10.1991, p. 9277-9281.

Research output: Contribution to journalArticle

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