Global aggregation of newly translated proteins in an Escherichia coli strain deficient of the chaperonin GroEL

Eli Chapman, George W. Farr, Renata Usaite, Krystyna Furtak, Wayne A. Fenton, Tapan K. Chaudhuri, Elise R. Hondorp, Rowena G. Matthews, Sharon G. Wolf, John R. Yates, Marc Pypaert, Arthur L. Horwich

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

In a newly isolated temperature-sensitive lethal Escherichia coli mutant affecting the chaperonin GroEL, we observed wholesale aggregation of newly translated proteins. After temperature shift, transcription, translation, and growth slowed over two to three generations, accompanied by filamentation and accretion (in ≈2% of cells) of paracrystalline arrays containing mutant chaperonin complex. A biochemically isolated inclusion body fraction contained the collective of abundant proteins of the bacterial cytoplasm as determined by SDS/PAGE and proteolysis/MS analyses. Pulse-chase experiments revealed that newly made proteins, but not preexistent ones, were recruited to this insoluble fraction. Although aggregation of "stringent" GroEL/GroES-dependent substrates may secondarily produce an "avalanche" of aggregation, the observations raise the possibility, supported by in vitro refolding experiments, that the widespread aggregation reflects that GroEL function supports the proper folding of a majority of newly translated polypeptides, not just the limited number indicated by interaction studies and in vitro experiments.

Original languageEnglish (US)
Pages (from-to)15800-15805
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume103
Issue number43
DOIs
StatePublished - Oct 24 2006
Externally publishedYes

Fingerprint

Chaperonins
Escherichia coli Proteins
Avalanches
Temperature
Bacterial Proteins
Inclusion Bodies
Proteolysis
Polyacrylamide Gel Electrophoresis
Cytoplasm
Proteins
Escherichia coli
Peptides
Growth
In Vitro Techniques

Keywords

  • Chaperone
  • Misfolding
  • Protein folding

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Global aggregation of newly translated proteins in an Escherichia coli strain deficient of the chaperonin GroEL. / Chapman, Eli; Farr, George W.; Usaite, Renata; Furtak, Krystyna; Fenton, Wayne A.; Chaudhuri, Tapan K.; Hondorp, Elise R.; Matthews, Rowena G.; Wolf, Sharon G.; Yates, John R.; Pypaert, Marc; Horwich, Arthur L.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, No. 43, 24.10.2006, p. 15800-15805.

Research output: Contribution to journalArticle

Chapman, E, Farr, GW, Usaite, R, Furtak, K, Fenton, WA, Chaudhuri, TK, Hondorp, ER, Matthews, RG, Wolf, SG, Yates, JR, Pypaert, M & Horwich, AL 2006, 'Global aggregation of newly translated proteins in an Escherichia coli strain deficient of the chaperonin GroEL', Proceedings of the National Academy of Sciences of the United States of America, vol. 103, no. 43, pp. 15800-15805. https://doi.org/10.1073/pnas.0607534103
Chapman, Eli ; Farr, George W. ; Usaite, Renata ; Furtak, Krystyna ; Fenton, Wayne A. ; Chaudhuri, Tapan K. ; Hondorp, Elise R. ; Matthews, Rowena G. ; Wolf, Sharon G. ; Yates, John R. ; Pypaert, Marc ; Horwich, Arthur L. / Global aggregation of newly translated proteins in an Escherichia coli strain deficient of the chaperonin GroEL. In: Proceedings of the National Academy of Sciences of the United States of America. 2006 ; Vol. 103, No. 43. pp. 15800-15805.
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