Global and local genome mapping in Arabidopsis thaliana by using recombinant inbred lines and random amplified polymorphic DNAs

Robert S. Reiter, John G.K. Williams, Kenneth A. Feldmann, J. Antoni Rafalski, Scott V. Tingey, Pablo A. Scolnik

Research output: Contribution to journalArticle

315 Scopus citations

Abstract

A population of Arabidopsis thaliana recombinant inbred lines was constructed and used to develop a high-density genetic linkage map containing 252 random amplified polymorphic DNA markers and 60 previously mapped restriction fragment length polymorphisms. Linkage groups were correlated to the classical genetic map by inclusion of nine phenotypic markers in the mapping cross. We also applied a technique for local mapping that allows targeting of markers to a selected genome region by pooling DNA from recombinant inbred lines based on their genotype. We conclude that random amplified polymorphic DNAs, used in conjunction with a recombinant inbred population, can facilitate the genetic and physical characterization of the Arabidopsis genome and that this method is generally applicable to other organisms for which appropriate populations either are available or can be developed.

Original languageEnglish (US)
Pages (from-to)1477-1481
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume89
Issue number4
DOIs
StatePublished - Feb 15 1992

Keywords

  • Chromosome walking
  • Genetic map
  • Restriction fragment length polymorphisms

ASJC Scopus subject areas

  • General

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