Global cell surface conformational shift mediated by a Candida albicans adhesin

Jason M. Rauceo, Nand K. Gaur, Kyeng Gea Lee, John E. Edwards, Stephen A Klotz, Peter N. Lipke

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Candida albicans maintains both commensal and pathogenic states in humans. Both states are dependent on cell surface-expressed adhesins, including those of the Als family. Heterologous expression of Als5p at the surface of Saccharomyces cerevisiae results in Als5p-mediated adhesion to various ligands, followed by formation of multicellular aggregates. Following adhesion of one region of the cell to fibronectin-coated beads, the entire surface of the cells became competent to mediate cell-cell aggregation. Aggregates formed in the presence of metabolic inhibitors or signal transduction inhibitors but were reduced in the presence of 8-anilino-1-naphthalene-sulfonic acid (ANS) or Congo Red (CR), perturbants that inhibit protein structural transitions. These perturbants also inhibited aggregation of C. albicans. An increase in ANS fluorescence, which accompanied Als-dependent cellular adhesion, indicated an increase in cell surface hydrophobicity. In addition, C. albicans and Als5p-expressing S. cerevisiae showed an aggregation-induced birefringence indicative of order on the cell surface. The increase in birefringence did not occur in the presence of the aggregation disruptants ANS and CR. These results suggest a model for Als5p-mediated aggregation in which an adhesion-triggered change in the conformation of Als5p propagates around the cell surface, forming ordered aggregation-competent regions.

Original languageEnglish (US)
Pages (from-to)4948-4955
Number of pages8
JournalInfection and Immunity
Volume72
Issue number9
DOIs
StatePublished - Sep 2004

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lissamine rhodamine B
Sulfonic Acids
Candida albicans
Birefringence
Congo Red
Saccharomyces cerevisiae
Cell Aggregation
Candida albicans ALA1 protein
Hydrophobic and Hydrophilic Interactions
Fibronectins
Signal Transduction
Fluorescence
Ligands
naphthalene
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Global cell surface conformational shift mediated by a Candida albicans adhesin. / Rauceo, Jason M.; Gaur, Nand K.; Lee, Kyeng Gea; Edwards, John E.; Klotz, Stephen A; Lipke, Peter N.

In: Infection and Immunity, Vol. 72, No. 9, 09.2004, p. 4948-4955.

Research output: Contribution to journalArticle

Rauceo, Jason M. ; Gaur, Nand K. ; Lee, Kyeng Gea ; Edwards, John E. ; Klotz, Stephen A ; Lipke, Peter N. / Global cell surface conformational shift mediated by a Candida albicans adhesin. In: Infection and Immunity. 2004 ; Vol. 72, No. 9. pp. 4948-4955.
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