Global characterization of cell-specific gene expression through fluorescence-activated sorting of nuclei

Changqing Zhang, Roger A. Barthelson, Georgina M. Lambert, David W Galbraith

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

We describe a simple and highly effective means for global identification of genes that are expressed within specific cell types within complex tissues. It involves transgenic expression of nuclear-targeted green fluorescent protein in a cell-type-specific manner. The fluorescent nuclei are then purified from homogenates by fluorescence-activated sorting, and the RNAs employed as targets for microarray hybridization. We demonstrate the validity of the approach through the identification of 12 genes that are selectively expressed in phloem.

Original languageEnglish (US)
Pages (from-to)30-40
Number of pages11
JournalPlant Physiology
Volume147
Issue number1
DOIs
StatePublished - May 2008

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sorting
Fluorescence
RNA Transport
fluorescence
Phloem
Gene Expression
microarray technology
gene expression
Green Fluorescent Proteins
green fluorescent protein
phloem
Genes
genes
RNA
cells
tissues

ASJC Scopus subject areas

  • Plant Science
  • Genetics
  • Physiology

Cite this

Global characterization of cell-specific gene expression through fluorescence-activated sorting of nuclei. / Zhang, Changqing; Barthelson, Roger A.; Lambert, Georgina M.; Galbraith, David W.

In: Plant Physiology, Vol. 147, No. 1, 05.2008, p. 30-40.

Research output: Contribution to journalArticle

Zhang, Changqing ; Barthelson, Roger A. ; Lambert, Georgina M. ; Galbraith, David W. / Global characterization of cell-specific gene expression through fluorescence-activated sorting of nuclei. In: Plant Physiology. 2008 ; Vol. 147, No. 1. pp. 30-40.
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