Global distribution of white spot syndrome virus genotypes determined using a novel genotyping assay

J. Oakey, C. Smith, D. Underwood, M. Afsharnasab, V. Alday-Sanz, A. Dhar, S. Sivakumar, A. S. Sahul Hameed, K. Beattie, A. Crook

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

White spot disease, caused by infection with white spot syndrome virus (WSSV), is a serious panzootic affecting prawn aquaculture. The disease has spread rapidly around the prawn-culturing regions of the world through a number of previously identified mechanisms. The ability to distinguish and trace strains of WSSV is of great benefit to identify, and then limit, the translocation routes of the disease. Here, we describe a novel genotyping method using 34 short tandem repeat regions of the viral genome concurrently. This technique is highly sensitive to strain differences when compared to previous methods. The efficacy of the described method is demonstrated by testing WSSV isolates from around the globe, showing regional genotypic differences. The differences in the genotypes were used to create a global minimum spanning network, and in most cases the observed relationships were substantiated with verification of transboundary movement. This novel panel of STR markers will provide a valuable epidemiological tool for white spot disease. We have applied this to an outbreak of the disease in Queensland, Australia, that occurred in 2016. While the results indicate that the source of this outbreak currently remains cryptic, the analyses have provided valuable insights with which to further study the origins of the strains involved.

Original languageEnglish (US)
JournalArchives of virology
DOIs
StateAccepted/In press - Jan 1 2019

Fingerprint

White spot syndrome virus 1
Genotype
Dental Caries
Disease Outbreaks
Aquaculture
Queensland
Viral Genome
Microsatellite Repeats
Infection

ASJC Scopus subject areas

  • Virology

Cite this

Global distribution of white spot syndrome virus genotypes determined using a novel genotyping assay. / Oakey, J.; Smith, C.; Underwood, D.; Afsharnasab, M.; Alday-Sanz, V.; Dhar, A.; Sivakumar, S.; Sahul Hameed, A. S.; Beattie, K.; Crook, A.

In: Archives of virology, 01.01.2019.

Research output: Contribution to journalArticle

Oakey, J, Smith, C, Underwood, D, Afsharnasab, M, Alday-Sanz, V, Dhar, A, Sivakumar, S, Sahul Hameed, AS, Beattie, K & Crook, A 2019, 'Global distribution of white spot syndrome virus genotypes determined using a novel genotyping assay', Archives of virology. https://doi.org/10.1007/s00705-019-04265-2
Oakey, J. ; Smith, C. ; Underwood, D. ; Afsharnasab, M. ; Alday-Sanz, V. ; Dhar, A. ; Sivakumar, S. ; Sahul Hameed, A. S. ; Beattie, K. ; Crook, A. / Global distribution of white spot syndrome virus genotypes determined using a novel genotyping assay. In: Archives of virology. 2019.
@article{a852be0eaa0f4331962a62c535b8b1c0,
title = "Global distribution of white spot syndrome virus genotypes determined using a novel genotyping assay",
abstract = "White spot disease, caused by infection with white spot syndrome virus (WSSV), is a serious panzootic affecting prawn aquaculture. The disease has spread rapidly around the prawn-culturing regions of the world through a number of previously identified mechanisms. The ability to distinguish and trace strains of WSSV is of great benefit to identify, and then limit, the translocation routes of the disease. Here, we describe a novel genotyping method using 34 short tandem repeat regions of the viral genome concurrently. This technique is highly sensitive to strain differences when compared to previous methods. The efficacy of the described method is demonstrated by testing WSSV isolates from around the globe, showing regional genotypic differences. The differences in the genotypes were used to create a global minimum spanning network, and in most cases the observed relationships were substantiated with verification of transboundary movement. This novel panel of STR markers will provide a valuable epidemiological tool for white spot disease. We have applied this to an outbreak of the disease in Queensland, Australia, that occurred in 2016. While the results indicate that the source of this outbreak currently remains cryptic, the analyses have provided valuable insights with which to further study the origins of the strains involved.",
author = "J. Oakey and C. Smith and D. Underwood and M. Afsharnasab and V. Alday-Sanz and A. Dhar and S. Sivakumar and {Sahul Hameed}, {A. S.} and K. Beattie and A. Crook",
year = "2019",
month = "1",
day = "1",
doi = "10.1007/s00705-019-04265-2",
language = "English (US)",
journal = "Archives of Virology",
issn = "0304-8608",
publisher = "Springer Wien",

}

TY - JOUR

T1 - Global distribution of white spot syndrome virus genotypes determined using a novel genotyping assay

AU - Oakey, J.

AU - Smith, C.

AU - Underwood, D.

AU - Afsharnasab, M.

AU - Alday-Sanz, V.

AU - Dhar, A.

AU - Sivakumar, S.

AU - Sahul Hameed, A. S.

AU - Beattie, K.

AU - Crook, A.

PY - 2019/1/1

Y1 - 2019/1/1

N2 - White spot disease, caused by infection with white spot syndrome virus (WSSV), is a serious panzootic affecting prawn aquaculture. The disease has spread rapidly around the prawn-culturing regions of the world through a number of previously identified mechanisms. The ability to distinguish and trace strains of WSSV is of great benefit to identify, and then limit, the translocation routes of the disease. Here, we describe a novel genotyping method using 34 short tandem repeat regions of the viral genome concurrently. This technique is highly sensitive to strain differences when compared to previous methods. The efficacy of the described method is demonstrated by testing WSSV isolates from around the globe, showing regional genotypic differences. The differences in the genotypes were used to create a global minimum spanning network, and in most cases the observed relationships were substantiated with verification of transboundary movement. This novel panel of STR markers will provide a valuable epidemiological tool for white spot disease. We have applied this to an outbreak of the disease in Queensland, Australia, that occurred in 2016. While the results indicate that the source of this outbreak currently remains cryptic, the analyses have provided valuable insights with which to further study the origins of the strains involved.

AB - White spot disease, caused by infection with white spot syndrome virus (WSSV), is a serious panzootic affecting prawn aquaculture. The disease has spread rapidly around the prawn-culturing regions of the world through a number of previously identified mechanisms. The ability to distinguish and trace strains of WSSV is of great benefit to identify, and then limit, the translocation routes of the disease. Here, we describe a novel genotyping method using 34 short tandem repeat regions of the viral genome concurrently. This technique is highly sensitive to strain differences when compared to previous methods. The efficacy of the described method is demonstrated by testing WSSV isolates from around the globe, showing regional genotypic differences. The differences in the genotypes were used to create a global minimum spanning network, and in most cases the observed relationships were substantiated with verification of transboundary movement. This novel panel of STR markers will provide a valuable epidemiological tool for white spot disease. We have applied this to an outbreak of the disease in Queensland, Australia, that occurred in 2016. While the results indicate that the source of this outbreak currently remains cryptic, the analyses have provided valuable insights with which to further study the origins of the strains involved.

UR - http://www.scopus.com/inward/record.url?scp=85067672419&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85067672419&partnerID=8YFLogxK

U2 - 10.1007/s00705-019-04265-2

DO - 10.1007/s00705-019-04265-2

M3 - Article

C2 - 31131427

AN - SCOPUS:85067672419

JO - Archives of Virology

JF - Archives of Virology

SN - 0304-8608

ER -