TY - JOUR
T1 - Global variation in the piggyBac-like element of pink bollworm, Pectinophora gossypiella
AU - Wang, Jianjun
AU - Simmons, Gregory S.
AU - Miller, Thomas A.
AU - Tabashnik, Bruce E.
AU - Park, Yoonseong
N1 - Funding Information:
We thank Dr. John Goolsby for providing P. endema from Australia and Dr. G. S. Deol (Punjab Agricultural University) for providing the samples from India, and Devika Unnithan for assistance in obtaining samples, and Dr. Kun Yan Zhu for reviewing an earlier version of this manuscript. This work was supported by a USDA-APHIS Cooperative agreement award and by the National Natural Science Foundation of China under grant nos. 03-8100-0879-CA and 30671375 . This paper is Contribution No. 11-116-J from the Kansas Agricultural Experiment Station, Manhattan, Kansas. Strong support for this work from the California Cotton Pest Control Board is appreciated.
PY - 2011/3
Y1 - 2011/3
N2 - The piggyBac transposable element, originally discovered in the cabbage looper, Trichoplusia ni, has been used widely in genetic engineering of insects including the pink bollworm, Pectinophora gossypiella, a major lepidopteran pest of cotton. Previously, we identified an intact copy of a piggyBac-like element (PLE) in pink bollworm, designated as PgPLE1.1. Here we report global variation in the occurrence and sequence of PgPLE1.1 and its flanking sequences. Low to high frequency of the PgPLE1.1 insertion was observed in populations from USA, Mexico, China, India, and Israel, while there is no PgPLE1.1 insertion in the populations from Australia. Investigation of the five haplotypes of PgPLE1.1, their frequency, and the flanking sequences of PgPLE1.1 revealed significant differences of the populations from Australia and China compared to other global populations, although recent occurrences of extensive gene flows among global populations were evident.
AB - The piggyBac transposable element, originally discovered in the cabbage looper, Trichoplusia ni, has been used widely in genetic engineering of insects including the pink bollworm, Pectinophora gossypiella, a major lepidopteran pest of cotton. Previously, we identified an intact copy of a piggyBac-like element (PLE) in pink bollworm, designated as PgPLE1.1. Here we report global variation in the occurrence and sequence of PgPLE1.1 and its flanking sequences. Low to high frequency of the PgPLE1.1 insertion was observed in populations from USA, Mexico, China, India, and Israel, while there is no PgPLE1.1 insertion in the populations from Australia. Investigation of the five haplotypes of PgPLE1.1, their frequency, and the flanking sequences of PgPLE1.1 revealed significant differences of the populations from Australia and China compared to other global populations, although recent occurrences of extensive gene flows among global populations were evident.
KW - Pectinophora gossypiella
KW - PiggyBac
KW - Pink bollworm
KW - Population genetics
KW - Transposon
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U2 - 10.1016/j.aspen.2010.09.006
DO - 10.1016/j.aspen.2010.09.006
M3 - Article
AN - SCOPUS:79551597496
VL - 14
SP - 131
EP - 135
JO - Journal of Asia-Pacific Entomology
JF - Journal of Asia-Pacific Entomology
SN - 1226-8615
IS - 1
ER -