Glomerular accumulation of monocytes and macrophages in experimental glomerulonephritis associated with Trypanosoma rhodesiense infection

Raymond B Nagle, S. Dong, L. L. Janacek, J. M. Guillot, H. B. Lindsley

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Abstract

Experimental infection in rabbits with a human isolate of Trypanosoma rhodesiense led to the formation of circulating immune complexes and glomerulonephritis. Granular deposits of C3 and lesser amounts of IgM and IgG were seen deposited in the glomeruli in a primarily mesangial pattern. The glomeruli became hypercellular beginning on day 7. This was associated with diffuse swelling and vacuolation of endothelial cells with focal loss of fenestrae, as well as vacuolation of mesangial cells processes protruding into the capillary lumina. The hypercellularity became maximal on day 21 and was accompanied by proteinuria and increased tubular hyaline droplets. The hypercellularity was in large part due to the accumulation of monocytes as demonstrated by nonspecific cytoplasmic esterase stains. Counts of the number of monocytic cellular profiles per glomerulus showed that maximal numbers were reached on the 21st day of the infection. Ultrastructural examination confirmed the presence of monocytes within capillary lumina and macrophages within mesangial regions. Electron-dense deposits were rarely seen by transmission electron microscopy, and the heavy granular deposits of IgM and C3 observed by immunofluorescence were attributed to ingested proteins within macrophages. This study implies an active role for monocytes and macrophages in immune complex-mediated glomerulonephritis.

Original languageEnglish (US)
Pages (from-to)365-376
Number of pages12
JournalLaboratory Investigation
Volume46
Issue number4
StatePublished - 1982

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Trypanosoma brucei rhodesiense
Glomerulonephritis
Monocytes
Macrophages
Antigen-Antibody Complex
Immunoglobulin M
Infection
Carboxylesterase
Hyalin
Mesangial Cells
Transmission Electron Microscopy
Proteinuria
Fluorescent Antibody Technique
Coloring Agents
Endothelial Cells
Immunoglobulin G
Electrons
Rabbits
Proteins

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Glomerular accumulation of monocytes and macrophages in experimental glomerulonephritis associated with Trypanosoma rhodesiense infection. / Nagle, Raymond B; Dong, S.; Janacek, L. L.; Guillot, J. M.; Lindsley, H. B.

In: Laboratory Investigation, Vol. 46, No. 4, 1982, p. 365-376.

Research output: Contribution to journalArticle

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AU - Lindsley, H. B.

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