Glucocorticoid activity detected by in vivo zebrafish assay and in vitro glucocorticoid receptor bioassay at environmental relevant concentrations

Qiyu Chen, Ai Jia, Shane A Snyder, Zhiyuan Gong, Siew Hong Lam

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Glucocorticoids are pharmaceutical contaminants of emerging concern due to their incomplete removal during wastewater treatment, increased presence in aquatic environment and their biological potency. The zebrafish is a popular model for aquatic toxicology and environmental risk assessment. This study aimed to determine if glucocorticoids at environmental concentrations would perturb expression of selected glucocorticoid-responsive genes in zebrafish and to investigate their potentials as an in vivo zebrafish assay in complementing in vitro glucocorticoid receptor bioassay. The relative expression of eleven glucocorticoid-responsive genes in zebrafish larvae and liver of adult male zebrafish exposed to three representative glucocorticoids (dexamethasone, prednisolone and triamcinolone) was determined. The expression of pepck, baiap2 and pxr was up-regulated in zebrafish larvae and the expression of baiap2, pxr and mmp-2 was up-regulated in adult zebrafish exposed to glucocorticoids at concentrations equivalent to total glucocorticoids reported in environmental samples. The responsiveness of the specific genes were sufficiently robust in zebrafish larvae exposed to a complex environmental sample detected with in vitro glucocorticoid activity equivalent to 478 pM dexamethasone (DEX-EQ) and confirmed to contain low concentration (0.2 ng/L or less) of the targeted glucocorticoids, and possibly other glucocorticoid-active compounds. The findings provided in vivo relevance to the in vitro glucocorticoid activity and suggested that the environmental sample can perturb glucocorticoid-responsive genes in its original, or half the diluted, concentration as may be found in the environment. The study demonstrated the important complementary roles of in vivo zebrafish and in vitro bioassays coupled with analytical chemistry in monitoring environmental glucocorticoid contaminants.

Original languageEnglish (US)
Pages (from-to)1162-1169
Number of pages8
JournalChemosphere
Volume144
DOIs
StatePublished - Feb 1 2016

Fingerprint

Bioassay
Glucocorticoid Receptors
Zebrafish
Biological Assay
Glucocorticoids
bioassay
Assays
gene
larva
pollutant
toxicology
environmental monitoring
Genes
aquatic environment
Larva
drug
In Vitro Techniques
in vivo assay
Dexamethasone
Ecotoxicology

Keywords

  • Environmental glucocorticoid contaminants
  • Gene expression
  • In vitro glucocorticoid receptor bioassay
  • In vivo zebrafish assay

ASJC Scopus subject areas

  • Environmental Chemistry
  • Chemistry(all)

Cite this

Glucocorticoid activity detected by in vivo zebrafish assay and in vitro glucocorticoid receptor bioassay at environmental relevant concentrations. / Chen, Qiyu; Jia, Ai; Snyder, Shane A; Gong, Zhiyuan; Lam, Siew Hong.

In: Chemosphere, Vol. 144, 01.02.2016, p. 1162-1169.

Research output: Contribution to journalArticle

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