Glucocorticoid receptor in clonal osteosarcoma cell lines: A novel system for investigating bone active hormones

Mark R Haussler, S. C. Manolagas, L. J. Deftos

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Cytosol from cultured osteogenic sarcoma cells contains a high affinity (Kd=1.4×10-9M) triamcinolone(TRM) binding component which adsorbs to DNA-cellulose. The TRM binding molecule sediments at 4S in 0.3M KC1-sucrose gradients, but appears as a 6-7S species when sodium molybdate is present. The concentration of TRM binder is 100fmol/mg cytosol protein (∼ 60,000 receptors/cell) and it displays the classical hierarchy of glucocorticoid potency in competition experiments. Thus, osteogenic sarcoma possesses a typical glucocorticoid receptor. This osteoblast-like malignant bone cell should serve as a model for studying the actions and interactions of glucocorticoids, 1,25-dihydroxyvitamin D and other bone active hormones.

Original languageEnglish (US)
Pages (from-to)373-380
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume94
Issue number1
DOIs
StatePublished - May 14 1980
Externally publishedYes

Fingerprint

Triamcinolone
Glucocorticoid Receptors
Osteosarcoma
Bone
Cells
Hormones
Bone and Bones
Cell Line
Cytosol
Glucocorticoids
Osteoblasts
Binders
Sucrose
Sediments
Molecules
Proteins
Experiments

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Glucocorticoid receptor in clonal osteosarcoma cell lines : A novel system for investigating bone active hormones. / Haussler, Mark R; Manolagas, S. C.; Deftos, L. J.

In: Biochemical and Biophysical Research Communications, Vol. 94, No. 1, 14.05.1980, p. 373-380.

Research output: Contribution to journalArticle

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