Glucocorticoid receptor in clonal osteosarcoma cell lines: A novel system for investigating bone active hormones

M. R. Haussler, S. C. Manolagas, L. J. Deftos

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

Cytosol from cultured osteogenic sarcoma cells contains a high affinity (Kd=1.4×10-9M) triamcinolone(TRM) binding component which adsorbs to DNA-cellulose. The TRM binding molecule sediments at 4S in 0.3M KC1-sucrose gradients, but appears as a 6-7S species when sodium molybdate is present. The concentration of TRM binder is 100fmol/mg cytosol protein (∼ 60,000 receptors/cell) and it displays the classical hierarchy of glucocorticoid potency in competition experiments. Thus, osteogenic sarcoma possesses a typical glucocorticoid receptor. This osteoblast-like malignant bone cell should serve as a model for studying the actions and interactions of glucocorticoids, 1,25-dihydroxyvitamin D and other bone active hormones.

Original languageEnglish (US)
Pages (from-to)373-380
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume94
Issue number1
DOIs
StatePublished - May 14 1980

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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