Cytosol from cultured osteogenic sarcoma cells contains a high affinity (Kd=1.4×10-9M) triamcinolone(TRM) binding component which adsorbs to DNA-cellulose. The TRM binding molecule sediments at 4S in 0.3M KC1-sucrose gradients, but appears as a 6-7S species when sodium molybdate is present. The concentration of TRM binder is 100fmol/mg cytosol protein (∼ 60,000 receptors/cell) and it displays the classical hierarchy of glucocorticoid potency in competition experiments. Thus, osteogenic sarcoma possesses a typical glucocorticoid receptor. This osteoblast-like malignant bone cell should serve as a model for studying the actions and interactions of glucocorticoids, 1,25-dihydroxyvitamin D and other bone active hormones.
|Original language||English (US)|
|Number of pages||8|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - May 14 1980|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology