Glucose transporter protein content and glucose transport capacity in rat skeletal muscles

E. J. Henriksen, R. E. Bourey, K. J. Rodnick, L. Koranyi, M. A. Permutt, J. O. Holloszy

Research output: Contribution to journalArticle

369 Scopus citations

Abstract

The relationships among fiber type, glucose transporter (GLUT-4) protein content, and glucose transport activity stimulated maximally with insulin and/or contractile activity were studied by use of the rat epitrochlearis (15% type I-20% type II2a-65% type IIb), soleus (84-16-0%), extensor digitorum longus (EDL, 3-57-40%), and flexor digitorum brevis (FDB, 7-92-1%) muscles. Insulin-stimulated 2-deoxy-D-glucose (2-DG) uptake was greatest in the soleus, followed (in order) by the FDB, EDL, and epitrochlearis. On the other hand, contractile activity induced the greatest increase in 2-DG uptake in the FDB, followed by the EDL, soleus, and epitrochlearis. The effects of insulin and contractile activity on 2-DG uptake were additive in all the muscle preparations, with the relative rates being FDB > soleus > EDL > epitrochlearis. Quantitation of the GLUT-4 protein content with the antiserum R820 showed the following pattern: FDB > soleus > EDL > epitrochlearis. Linear regression analysis showed that whereas a relatively low and nonsignificant correlation existed between GLUT-4 protein content and 2-DG uptake stimulated by insulin alone, significant correlations existed between GLUT-4 protein content and 2-DG uptake stimulated either by contractions alone (r = 0.950) or by insulin and contractions in combination (r = 0.992). These results suggest that the differences in maximally stimulated glucose transport activity among the three fiber types may be related to differences in their content of GLUT-4 protein.

Original languageEnglish (US)
Pages (from-to)E593-E598
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume259
Issue number4 22-4
DOIs
StatePublished - 1990

Keywords

  • 2-deoxy-D-glucose uptake
  • antiserum R820
  • contractile activity
  • glucose transporter-4 protein
  • insulin
  • messenger ribonucleic acid

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Physiology (medical)

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