Glucose uptake in porcine carotid artery: Relation to alterations in active Na+-K+ transport

Ron Lynch, R. J. Paul

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4 Citations (Scopus)

Abstract

In vascular smooth muscle (VSM), aerobic lactate production can account for as much as 30% of the basal rate of ATP production. Generally, glucose transport is thought to be the rate-limiting step for glycolysis in unstimulated VSM. In this work we provide evidence that the intracellular concentration of glucose is negligible in porcine carotid artery, indicating that glucose transport is rate limiting for its utilization. Since aerobic glycolysis appears to be coupled to active Na+-K+ transport in this tissue, we examined the effects of altering ion transport on glucose transport. Glucose uptake and 3-O-methyl-D-glucose transport were accelerated, though intracellular glucose remained negligible in artery rings that were incubated with 80 mM KCl, which is known to stimulate active Na+-K+ transport, as well as aerobic glycolysis and mechanical activity. On the other hand, inhibitors of active Na+-K+ transport (ouabain, Na+-free media), which also elicit mechanical activity, had little effect on sugar transport but significantly inhibited aerobic glycolysis and caused an intracellular accumulation of glucose. Our results indicate the following: 1) that glucose transport is regulated in VSM; 2) that the intracellular concentration of Ca2+ does not appear to regulate sugar transport, since changes in glucose and 3-O-methyl-D-glucose transport are not always seen in association with increased mechanical activity, and 3) that the decrease in aerobic glycolysis associated with the inhibition of active Na+-K+ transport is not due to a decrease in glucose transport but rather to an inhibition of glucose utilization.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume16
Issue number3
StatePublished - 1984
Externally publishedYes

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Carotid Arteries
Swine
Glucose
Glycolysis
3-O-Methylglucose
Vascular Smooth Muscle
Muscle
Sugars
Ouabain
Ion Transport
Lactic Acid
Adenosine Triphosphate
Arteries
Ions
Tissue

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

Cite this

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abstract = "In vascular smooth muscle (VSM), aerobic lactate production can account for as much as 30{\%} of the basal rate of ATP production. Generally, glucose transport is thought to be the rate-limiting step for glycolysis in unstimulated VSM. In this work we provide evidence that the intracellular concentration of glucose is negligible in porcine carotid artery, indicating that glucose transport is rate limiting for its utilization. Since aerobic glycolysis appears to be coupled to active Na+-K+ transport in this tissue, we examined the effects of altering ion transport on glucose transport. Glucose uptake and 3-O-methyl-D-glucose transport were accelerated, though intracellular glucose remained negligible in artery rings that were incubated with 80 mM KCl, which is known to stimulate active Na+-K+ transport, as well as aerobic glycolysis and mechanical activity. On the other hand, inhibitors of active Na+-K+ transport (ouabain, Na+-free media), which also elicit mechanical activity, had little effect on sugar transport but significantly inhibited aerobic glycolysis and caused an intracellular accumulation of glucose. Our results indicate the following: 1) that glucose transport is regulated in VSM; 2) that the intracellular concentration of Ca2+ does not appear to regulate sugar transport, since changes in glucose and 3-O-methyl-D-glucose transport are not always seen in association with increased mechanical activity, and 3) that the decrease in aerobic glycolysis associated with the inhibition of active Na+-K+ transport is not due to a decrease in glucose transport but rather to an inhibition of glucose utilization.",
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T2 - Relation to alterations in active Na+-K+ transport

AU - Lynch, Ron

AU - Paul, R. J.

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AB - In vascular smooth muscle (VSM), aerobic lactate production can account for as much as 30% of the basal rate of ATP production. Generally, glucose transport is thought to be the rate-limiting step for glycolysis in unstimulated VSM. In this work we provide evidence that the intracellular concentration of glucose is negligible in porcine carotid artery, indicating that glucose transport is rate limiting for its utilization. Since aerobic glycolysis appears to be coupled to active Na+-K+ transport in this tissue, we examined the effects of altering ion transport on glucose transport. Glucose uptake and 3-O-methyl-D-glucose transport were accelerated, though intracellular glucose remained negligible in artery rings that were incubated with 80 mM KCl, which is known to stimulate active Na+-K+ transport, as well as aerobic glycolysis and mechanical activity. On the other hand, inhibitors of active Na+-K+ transport (ouabain, Na+-free media), which also elicit mechanical activity, had little effect on sugar transport but significantly inhibited aerobic glycolysis and caused an intracellular accumulation of glucose. Our results indicate the following: 1) that glucose transport is regulated in VSM; 2) that the intracellular concentration of Ca2+ does not appear to regulate sugar transport, since changes in glucose and 3-O-methyl-D-glucose transport are not always seen in association with increased mechanical activity, and 3) that the decrease in aerobic glycolysis associated with the inhibition of active Na+-K+ transport is not due to a decrease in glucose transport but rather to an inhibition of glucose utilization.

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