Glutamatergic Neurokinin 3 Receptor Neurons in the Median Preoptic Nucleus Modulate Heat-Defense Pathways in Female Mice

Sally J. Krajewski-Hall, Filipa Miranda Dos Santos, Nathaniel T. McMullen, Elise M. Blackmore, Naomi E Rance

Research output: Contribution to journalArticle

Abstract

We have proposed that arcuate neurons coexpressing kisspeptin, neurokinin B, and dynorphin (KNDy neurons) contribute to hot flushes via projections to neurokinin 3 receptor (NK3R)-expressing neurons in the median preoptic nucleus (MnPO). To characterize the thermoregulatory role of MnPO NK3R neurons in female mice, we ablated these neurons using injections of saporin toxin conjugated to a selective NK3R agonist. Loss of MnPO NK3R neurons increased the core temperature (TCORE) during the light phase, with the frequency distributions indicating a regulated shift in the balance point. The increase in TCORE in the ablated mice occurred despite changes in the ambient temperature and regardless of estrogen status. We next determined whether an acute increase in ambient temperature or higher TCORE would induce Fos in preoptic enhanced green fluorescent protein (EGFP)-immunoreactive neurons in Tacr3-EGFP mice. Fos activation was increased in the MnPO but no induction of Fos was found in NK3R (EGFP-immunoreactive) neurons. Thus, MnPO NK3R neurons are not activated by warm thermosensors in the skin or viscera and are not warm-sensitive neurons. Finally, RNAscope was used to determine whether Tacr3 (NK3R) mRNA was coexpressed with vesicular glutamate transporter 2 or vesicular γ-aminobutyric acid (GABA) transporter mRNA, markers of glutamatergic and GABAergic neurotransmission, respectively. In the MnPO, 94% of NK3R neurons were glutamatergic, but in the adjacent medial preoptic area, 97% of NK3R neurons were GABAergic. Thus, NK3R neurons in the MnPO are glutamatergic and play a role in reducing TCORE but are not activated by warm thermal stimuli (internal or external). These findings suggest that KNDy neurons modulate thermosensory pathways for heat defense indirectly via a subpopulation of glutamatergic MnPO neurons that express NK3R.

Original languageEnglish (US)
Pages (from-to)803-816
Number of pages14
JournalEndocrinology
Volume160
Issue number4
DOIs
StatePublished - Apr 1 2019

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Neurokinin-3 Receptors
Preoptic Area
Hot Temperature
Neurons
Temperature
Vesicular Glutamate Transport Protein 2
Neurokinin B
Kisspeptins
GABA Plasma Membrane Transport Proteins
Aminobutyrates
Dynorphins
GABAergic Neurons
Messenger RNA
Viscera

ASJC Scopus subject areas

  • Endocrinology

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Glutamatergic Neurokinin 3 Receptor Neurons in the Median Preoptic Nucleus Modulate Heat-Defense Pathways in Female Mice. / Krajewski-Hall, Sally J.; Miranda Dos Santos, Filipa; McMullen, Nathaniel T.; Blackmore, Elise M.; Rance, Naomi E.

In: Endocrinology, Vol. 160, No. 4, 01.04.2019, p. 803-816.

Research output: Contribution to journalArticle

Krajewski-Hall, Sally J. ; Miranda Dos Santos, Filipa ; McMullen, Nathaniel T. ; Blackmore, Elise M. ; Rance, Naomi E. / Glutamatergic Neurokinin 3 Receptor Neurons in the Median Preoptic Nucleus Modulate Heat-Defense Pathways in Female Mice. In: Endocrinology. 2019 ; Vol. 160, No. 4. pp. 803-816.
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