Glutamine transport by human intestinal basolateral membrane vesicle

Fayez K Ghishan, Noushin Arab, Nada Bulus, Hamid Said, John Pietsch, Naji Abumrad

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

This study characterizes for the first time, by use of a well-validated technique, glutamine transport across human basolateral membrane vesicles. Glutamine transport represented uptake into an osmotically active intravesicular space without significant metabolism. Glutamine uptake was temperature- and pH-dependent with maximal uptake at pH 7.5, and it was mediated by sodium-dependent and -independent processes. The initial rate of uptake was linear up to 20 s, as depicted by the formula y (nmol/mg protein) = 0.0009X (s) - 0.0011 (r = 0.99). Kinetic analysis of glutamine uptake at concentrations between 0.01 and 0.3 mmol/L at 5 s under sodium and potassium gradients showed a maximal transport capacity (Vmax) of 0.39 ± 0.04 and 0.21 ± 0.02 nmol · mg protein-1 · 5 s-1 for sodium-dependent and -independent processes, respectively (p < 0.01 ). Km values were 0.17 ± 0.04 and 0.06 ± 0.2 mmol/L, respectively (p < 0.05). Glutamine uptake under the sodium-gradient condition was electrogenic whereas under the potassium-gradient it was electroneutral. Neutral amino acids inhibited both sodium-dependent and -independent processes. This study confirms and characterizes the presence of carrier-mediated glutamine uptake at the basolateral membranes of human enterocytes.

Original languageEnglish (US)
Pages (from-to)612-616
Number of pages5
JournalAmerican Journal of Clinical Nutrition
Volume51
Issue number4
StatePublished - Apr 1990
Externally publishedYes

Fingerprint

Glutamine
glutamine
uptake mechanisms
Sodium
Membranes
sodium
Potassium
Neutral Amino Acids
potassium
Enterocytes
enterocytes
Proteins
proteins
Temperature
kinetics
amino acids
metabolism
temperature

Keywords

  • Basolateral membranes
  • Enterocytes
  • Glutamine
  • Human

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science

Cite this

Ghishan, F. K., Arab, N., Bulus, N., Said, H., Pietsch, J., & Abumrad, N. (1990). Glutamine transport by human intestinal basolateral membrane vesicle. American Journal of Clinical Nutrition, 51(4), 612-616.

Glutamine transport by human intestinal basolateral membrane vesicle. / Ghishan, Fayez K; Arab, Noushin; Bulus, Nada; Said, Hamid; Pietsch, John; Abumrad, Naji.

In: American Journal of Clinical Nutrition, Vol. 51, No. 4, 04.1990, p. 612-616.

Research output: Contribution to journalArticle

Ghishan, FK, Arab, N, Bulus, N, Said, H, Pietsch, J & Abumrad, N 1990, 'Glutamine transport by human intestinal basolateral membrane vesicle', American Journal of Clinical Nutrition, vol. 51, no. 4, pp. 612-616.
Ghishan, Fayez K ; Arab, Noushin ; Bulus, Nada ; Said, Hamid ; Pietsch, John ; Abumrad, Naji. / Glutamine transport by human intestinal basolateral membrane vesicle. In: American Journal of Clinical Nutrition. 1990 ; Vol. 51, No. 4. pp. 612-616.
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