Glutathione effects on toxicity and uptake of mercuric chloride and sodium arsenite in rabbit renal cortical slices

C. A. Burton, K. Hatlelid, K. Divine, D. E. Carter, Q. Fernando, K. Brendel, A Jay Gandolfi

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The mechanism of renal uptake of nephrotoxic heavy metals such as HgCl2 and NaAsO2 is not clear. The metals are known to react with endogenous sulfhydryls such as glutathione (GSH), so metal-GSH conjugates may be delivered to the kidney. To study this possibility, renal cortical slices from male New Zealand white rabbits were incubated with 10-4 M HgCl2 or 10-3 M NaAsO2 ± stoichiometric amounts (1-3x) of GSH; or synthetic metal-GSH conjugates [10-4 M Hg(SG)2 or 10-3 M As(SG)3]. Incubations were performed at 37°C in DME-F12 buffer (95/5 O2/CO2) for 8 hr. Hg(SG)2 reduced slice K+/DNA content, as an indicator of viability, significantly less than HgCl2. As(SG)3 exhibited a 2-hr delay in K+/DNA content reduction compared to NaAsO2. This delay in toxicity was not correlated to changes in uptake. Arsenic and mercury accumulation, determined by proton-induced X-ray emission, were also identical between the metal salts and the metal-GSH conjugates. Exogenous GSH decreased HgCl2 cytotoxicity and was correlated to a decrease in Hg accumulation in the slice. Exogenous GSH had limited if any protective effects against cytotoxicity by NaAsO2 and a decrease in As accumulation was not observed. Complex metal-GSH interactions appear to exist and impact on the uptake and toxicity of these metals.

Original languageEnglish (US)
Pages (from-to)81-84
Number of pages4
JournalEnvironmental Health Perspectives
Volume103
Issue numberSUPPL. 1
StatePublished - 1995

Fingerprint

Mercuric Chloride
arsenite
Glutathione
Toxicity
Metals
chloride
sodium
Rabbits
toxicity
Kidney
metal
Cytotoxicity
Synthetic metals
DNA
Coordination Complexes
Arsenic
Heavy Metals
Mercury
Protons
Buffers

Keywords

  • Arsenite
  • Glutathione
  • Mercuric chloride
  • Metal accumulation
  • Metal toxicity
  • Metals
  • Nephrotoxicity
  • Tissue slices

ASJC Scopus subject areas

  • Environmental Science(all)
  • Environmental Chemistry
  • Public Health, Environmental and Occupational Health

Cite this

Burton, C. A., Hatlelid, K., Divine, K., Carter, D. E., Fernando, Q., Brendel, K., & Gandolfi, A. J. (1995). Glutathione effects on toxicity and uptake of mercuric chloride and sodium arsenite in rabbit renal cortical slices. Environmental Health Perspectives, 103(SUPPL. 1), 81-84.

Glutathione effects on toxicity and uptake of mercuric chloride and sodium arsenite in rabbit renal cortical slices. / Burton, C. A.; Hatlelid, K.; Divine, K.; Carter, D. E.; Fernando, Q.; Brendel, K.; Gandolfi, A Jay.

In: Environmental Health Perspectives, Vol. 103, No. SUPPL. 1, 1995, p. 81-84.

Research output: Contribution to journalArticle

Burton, CA, Hatlelid, K, Divine, K, Carter, DE, Fernando, Q, Brendel, K & Gandolfi, AJ 1995, 'Glutathione effects on toxicity and uptake of mercuric chloride and sodium arsenite in rabbit renal cortical slices', Environmental Health Perspectives, vol. 103, no. SUPPL. 1, pp. 81-84.
Burton CA, Hatlelid K, Divine K, Carter DE, Fernando Q, Brendel K et al. Glutathione effects on toxicity and uptake of mercuric chloride and sodium arsenite in rabbit renal cortical slices. Environmental Health Perspectives. 1995;103(SUPPL. 1):81-84.
Burton, C. A. ; Hatlelid, K. ; Divine, K. ; Carter, D. E. ; Fernando, Q. ; Brendel, K. ; Gandolfi, A Jay. / Glutathione effects on toxicity and uptake of mercuric chloride and sodium arsenite in rabbit renal cortical slices. In: Environmental Health Perspectives. 1995 ; Vol. 103, No. SUPPL. 1. pp. 81-84.
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