Gold nanorods targeted to delta opioid receptor

Plasmon-resonant contrast and photothermal agents

Kvar C. Black, Nathaniel D. Kirkpatrick, Timothy S. Troutman, Liping Xu, Josef Vagner, Robert J. Gillies, Jennifer K Barton, Urs Utzinger, Marek Romanowski

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Molecularly targeted gold nanorods were investigated for applications in both diagnostic imaging and disease treatment with cellular resolution. The nanorods were tested in two genetically engineered cell lines derived from the human colon carcinoma HCT-116, a model for studying ligand-receptor interactions. One of these lines was modified to express delta opioid receptor (δOR) and green fluorescent protein, whereas the other was receptor free and expressed a red fluorescent protein, to serve as the control. Deltorphin, a high-affinity ligand for δOR, was stably attached to the gold nanorods through a thiol-terminated linker. In a mixed population of cells, we demonstrated selective imaging and destruction of receptor-expressing cells while sparing those cells that did not express the receptor. The molecularly targeted nanorods can be used as an in vitro ligand-binding and cytotoxic treatment assay platform and could potentially be applied in vivo for diagnostic and therapeutic purposes with endoscopic technology.

Original languageEnglish (US)
Pages (from-to)50-57
Number of pages8
JournalMolecular Imaging
Volume7
Issue number1
DOIs
StatePublished - Jan 2008

Fingerprint

Nanotubes
delta Opioid Receptor
Nanorods
Gold
Contrast Media
nanorods
gold
Ligands
Cells
Proteins
Imaging techniques
ligands
Opioid Receptors
Diagnostic Imaging
Green Fluorescent Proteins
Sulfhydryl Compounds
proteins
Assays
Colon
Technology

ASJC Scopus subject areas

  • Engineering(all)
  • Radiology Nuclear Medicine and imaging
  • Biotechnology
  • Molecular Medicine
  • Biomedical Engineering
  • Condensed Matter Physics

Cite this

Gold nanorods targeted to delta opioid receptor : Plasmon-resonant contrast and photothermal agents. / Black, Kvar C.; Kirkpatrick, Nathaniel D.; Troutman, Timothy S.; Xu, Liping; Vagner, Josef; Gillies, Robert J.; Barton, Jennifer K; Utzinger, Urs; Romanowski, Marek.

In: Molecular Imaging, Vol. 7, No. 1, 01.2008, p. 50-57.

Research output: Contribution to journalArticle

Black, Kvar C. ; Kirkpatrick, Nathaniel D. ; Troutman, Timothy S. ; Xu, Liping ; Vagner, Josef ; Gillies, Robert J. ; Barton, Jennifer K ; Utzinger, Urs ; Romanowski, Marek. / Gold nanorods targeted to delta opioid receptor : Plasmon-resonant contrast and photothermal agents. In: Molecular Imaging. 2008 ; Vol. 7, No. 1. pp. 50-57.
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