Golgi biogenesis in Toxoplasma gondii

Laurence Pelletier, Charlene A. Stern, Marc Pypaert, David Sheff, Huân M. Ngô, Nitin Roper, Cynthia Y. He, Ke Hu, Derek Toomre, Isabelle Coppens, David S. Roos, Keith A Joiner, Graham Warren

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Abstract

Two models have been put forward to explain the growth of new Golgi during the cell cycle. The first suggests that a new Golgi grows out of the endoplasmic reticulum by de novo synthesis. The second suggests that a pre-existing Golgi is needed for the growth of a new one, that is, the Golgi is an autonomously replicating organelle. To resolve this issue, we have exploited the simplicity of the apicomplexan parasite Toxoplasma gondii, which has only a single Golgi stack. Here we show, by using video fluorescence microscopy and three-dimensional reconstructions of serial thin sections, that the Golgi grows by a process of lateral extension followed by medial fission. Further fission leads to the inheritance by each daughter of a pair of Golgi structures, which then coalesce to re-form a single Golgi. Our results indicate that new Golgi grow by autonomous duplication and raise the possibility that the Golgi is a paired structure that is analogous to centrioles.

Original languageEnglish (US)
Pages (from-to)548-552
Number of pages5
JournalNature
Volume418
Issue number6897
DOIs
Publication statusPublished - Aug 1 2002
Externally publishedYes

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Cite this

Pelletier, L., Stern, C. A., Pypaert, M., Sheff, D., Ngô, H. M., Roper, N., ... Warren, G. (2002). Golgi biogenesis in Toxoplasma gondii. Nature, 418(6897), 548-552. https://doi.org/10.1038/nature00946