gp72, the 72 kilodalton glycoprotein, is the membrane acceptor site for C3 on Trypanosoma cruzi epimastigotes

Keith A Joiner, S. Hieny, L. V. Kirchhoff, A. Sher

Research output: Contribution to journalArticle

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Abstract

We examined the interaction of complement component C3 with surface molecules on Trypanosoma cruzi. Five- to six-fold more C3 was bound to epimastigotes (Epi) than to metacyclic trypomastigotes (CMT) of strain M88. Epi and CMT were surface iodinated, then incubated in C8-deficient serum, and detergent lysates were applied to anti-C3 antibody that had been coupled to Sepharose. We found that 9.20-10.24% of applied 125I-Epi protein bound to anti-C3-sepharose, compared to 2.64% binding of 125I-CMT protein. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that C3 was attached to 125I-Epi protein by a covalent bond. Samples eluted from anti-C3-sepharose with hydroxylamine revealed a single, major, 72 kD band, suggesting that C3b attaches almost exclusively to the 72 kD glycoprotein of Epi by a hydroxylamine-susceptible ester bond. An antiserum was prepared from lysates of serum-treated Epi that had been affinity-purified on anti-C3-sepharose. This antiserum immunoprecipitated a single 72 kD component (gp72) from surface-iodinated Epi, and specifically recognized only gp72 from Epi in immunoblots. In contrast to the results with Epi, gp72 on CMT was not found to be an efficient acceptor molecule for C3 deposition. The results are the first to evaluate the acceptor site for C3 deposition on a parasite, and they show that gp72 on Epi, but not gp72 on CMT, serves as the preferential acceptor for C3 during antibody-independent alternative complement pathway activation.

Original languageEnglish (US)
Pages (from-to)1196-1212
Number of pages17
JournalJournal of Experimental Medicine
Volume161
Issue number5
StatePublished - 1985
Externally publishedYes

Fingerprint

Trypanosoma cruzi
Membrane Glycoproteins
Sepharose
Hydroxylamine
Immune Sera
Alternative Complement Pathway
Complement C3
Proteins
Serum
Sodium Dodecyl Sulfate
Detergents
Polyacrylamide Gel Electrophoresis
Anti-Idiotypic Antibodies
Glycoproteins
Esters
Parasites
Antibodies

ASJC Scopus subject areas

  • Immunology

Cite this

gp72, the 72 kilodalton glycoprotein, is the membrane acceptor site for C3 on Trypanosoma cruzi epimastigotes. / Joiner, Keith A; Hieny, S.; Kirchhoff, L. V.; Sher, A.

In: Journal of Experimental Medicine, Vol. 161, No. 5, 1985, p. 1196-1212.

Research output: Contribution to journalArticle

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