Graded regulation of cellular quiescence depth between proliferation and senescence by a lysosomal dimmer switch

Kotaro Fujimaki, Ruoyan Li, Hengyu Chen, Kimiko Della Croce, Hao Helen Zhang, Jianhua Xing, Fan Bai, Guang Yao

Research output: Contribution to journalArticle

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Abstract

The reactivation of quiescent cells to proliferate is fundamental to tissue repair and homeostasis in the body. Often referred to as the G0 state, quiescence is, however, not a uniform state but with graded depth. Shallow quiescent cells exhibit a higher tendency to revert to proliferation than deep quiescent cells, while deep quiescent cells are still fully reversible under physiological conditions, distinct from senescent cells. Cellular mechanisms underlying the control of quiescence depth and the connection between quiescence and senescence are poorly characterized, representing a missing link in our understanding of tissue homeostasis and regeneration. Here we measured transcriptome changes as rat embryonic fibroblasts moved from shallow to deep quiescence over time in the absence of growth signals. We found that lysosomal gene expression was significantly up-regulated in deep quiescence, and partially compensated for gradually reduced autophagy flux. Reducing lysosomal function drove cells progressively deeper into quiescence and eventually into a senescence-like irreversibly arrested state; increasing lysosomal function, by lowering oxidative stress, progressively pushed cells into shallower quiescence. That is, lysosomal function modulates graded quiescence depth between proliferation and senescence as a dimmer switch. Finally, we found that a gene-expression signature developed by comparing deep and shallow quiescence in fibroblasts can correctly classify a wide array of senescent and aging cell types in vitro and in vivo, suggesting that while quiescence is generally considered to protect cells from irreversible arrest of senescence, quiescence deepening likely represents a common transition path from cell proliferation to senescence, related to aging.

Original languageEnglish (US)
Pages (from-to)22624-22634
Number of pages11
JournalProceedings of the National Academy of Sciences of the United States of America
Volume116
Issue number45
DOIs
StatePublished - Nov 5 2019

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Transcriptome
Homeostasis
Fibroblasts
Cell Aging
Autophagy
Regeneration
Oxidative Stress
Cell Proliferation
Gene Expression
Growth
In Vitro Techniques

Keywords

  • Aging
  • Dormancy
  • Lysosome
  • Quiescence
  • Senescence

ASJC Scopus subject areas

  • General

Cite this

Graded regulation of cellular quiescence depth between proliferation and senescence by a lysosomal dimmer switch. / Fujimaki, Kotaro; Li, Ruoyan; Chen, Hengyu; Croce, Kimiko Della; Zhang, Hao Helen; Xing, Jianhua; Bai, Fan; Yao, Guang.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 116, No. 45, 05.11.2019, p. 22624-22634.

Research output: Contribution to journalArticle

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