Green roots: Photosynthesis and photoautotrophy in an underground plant organ

Hector E. Flores, Yao Ren Dai, Joel L Cuello, Ignacio E. Maldonado-Mendoza, Victor M. Loyola-Vargas

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

The potential for photosynthetic and photoautotrophic growth was studied in hairy root cultures of Asteraceae and Solanaceae species. Upon transfer to light, initially heterotrophic root cultures of Acmella oppositifolia and Datura innoxia greened rapidly, differentiated chloroplasts, and developed light-dependent CO2 fixation in the cortical cells. Photosynthetic potential was expressed in root cultures of all the Asteraceae genera examined (Acmella, Artemisia, Rudbeckia, Stevia, and Tagetes). Hairy roots of A. oppositifolia and D. innoxia were further adapted to photoautotrophy by growing in the presence of light and added CO2 (1-5%) and by direct or sequential transfers into media containing progressively lower sugar concentrations. The transition to photoautotrophy was accompanied by an increase in CO2 fixation and in the specific activity of 1,5-ribulose-bisphosphate carboxylase/oxygenase (Rubisco). During the adaptation of A. oppositifolia roots to photoautotrophy, the ratio of Rubisco to phosphoenol pyruvate carboxylase increased significantly, approaching that found in the leaves. The levels and patterns of alkaloids and polyacetylenes produced by Solanaceae and Asteraceae hairy roots, respectively, were dramatically altered in photomixotrophic and photoautotrophic cultures. Photoautotrophic roots of A. oppositifolia have been maintained in vitro for over 2 years.

Original languageEnglish (US)
Pages (from-to)363-371
Number of pages9
JournalPlant Physiology
Volume101
Issue number2
StatePublished - Feb 1993
Externally publishedYes

Fingerprint

Asteraceae
Photosynthesis
autotrophs
plant organs
Solanaceae
Ribulose-Bisphosphate Carboxylase
Oxygenases
photosynthesis
Light
Rudbeckia
Tagetes
Stevia
Polyacetylenes
Datura
Pyruvate Carboxylase
Artemisia
Chloroplasts
Alkaloids
ribulose-bisphosphate carboxylase
oxygenases

ASJC Scopus subject areas

  • Plant Science

Cite this

Flores, H. E., Dai, Y. R., Cuello, J. L., Maldonado-Mendoza, I. E., & Loyola-Vargas, V. M. (1993). Green roots: Photosynthesis and photoautotrophy in an underground plant organ. Plant Physiology, 101(2), 363-371.

Green roots : Photosynthesis and photoautotrophy in an underground plant organ. / Flores, Hector E.; Dai, Yao Ren; Cuello, Joel L; Maldonado-Mendoza, Ignacio E.; Loyola-Vargas, Victor M.

In: Plant Physiology, Vol. 101, No. 2, 02.1993, p. 363-371.

Research output: Contribution to journalArticle

Flores, HE, Dai, YR, Cuello, JL, Maldonado-Mendoza, IE & Loyola-Vargas, VM 1993, 'Green roots: Photosynthesis and photoautotrophy in an underground plant organ', Plant Physiology, vol. 101, no. 2, pp. 363-371.
Flores HE, Dai YR, Cuello JL, Maldonado-Mendoza IE, Loyola-Vargas VM. Green roots: Photosynthesis and photoautotrophy in an underground plant organ. Plant Physiology. 1993 Feb;101(2):363-371.
Flores, Hector E. ; Dai, Yao Ren ; Cuello, Joel L ; Maldonado-Mendoza, Ignacio E. ; Loyola-Vargas, Victor M. / Green roots : Photosynthesis and photoautotrophy in an underground plant organ. In: Plant Physiology. 1993 ; Vol. 101, No. 2. pp. 363-371.
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