Green tea compound in chemoprevention of cervical cancer

Changping Zou, Huaguang Liu, Jean M. Feugang, Zhengping Hao, Hsiao-Hui Chow, Francisco A Garcia

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Objectives: Human papillomavirus (HPV) infection is closely associated with the development of more than 95% of cervical cancer. Clinical trials using several chemopreventive agents are underway, but results are inconclusive. Most agents used in trials inhibited the growth ofcancer cells invitro, and about halfof patients had some degree ofclinical responses; however, the therapeutic effect was confounded by high rates of spontaneous regression and relapse. The selection of nontoxic agents especially food, beverage, and natural products that suppress oncogenic HPV, inhibit malignant transformation, and can additionally be used long term may be important for cervical cancer prevention. Methods: We evaluated green tea compound (epigallocatechin gallate and polyphenols E) effects on immortalized cervical epithelial and cervical cancer cells. HPV-immortalized cervical epithelial cells, TCL1, and HPV-positive cervical cancer cells, Me180 and HeLa, were used in the study. The effects of green tea compounds on cell growth, apoptosis, cell cycle, and gene expression were examined and characterized. Results: Both epigallocatechin gallate and polyphenols E inhibited immortalized cervical epithelial and cancer cell growth. Apoptosis induction and cell cycle changes were observed in a dose-dependent manner. Western blot analysis of apoptosis-related proteins, p53 and p21, showed dose-dependent increase, whereas p27 was not affected. HPV-E7 protein expression was decreased by green tea compounds. Conclusions: This study provides information on the potential mechanisms of action of green tea compounds in suppression of HPV-related cervical cells, and it will enable us to assess the feasibility of using these agents.

Original languageEnglish (US)
Pages (from-to)617-624
Number of pages8
JournalInternational Journal of Gynecological Cancer
Volume20
Issue number4
DOIs
StatePublished - May 2010

Fingerprint

Chemoprevention
Tea
Uterine Cervical Neoplasms
Polyphenols
Apoptosis
Papillomavirus E7 Proteins
Growth
Epithelial Cells
Food and Beverages
cdc Genes
Papillomavirus Infections
Therapeutic Uses
Biological Products
HeLa Cells
Action Potentials
Cell Cycle
Western Blotting
Clinical Trials
Gene Expression
Recurrence

Keywords

  • Cervical cancer
  • HPV-E7
  • p21
  • p53

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Oncology

Cite this

Green tea compound in chemoprevention of cervical cancer. / Zou, Changping; Liu, Huaguang; Feugang, Jean M.; Hao, Zhengping; Chow, Hsiao-Hui; Garcia, Francisco A.

In: International Journal of Gynecological Cancer, Vol. 20, No. 4, 05.2010, p. 617-624.

Research output: Contribution to journalArticle

Zou, Changping ; Liu, Huaguang ; Feugang, Jean M. ; Hao, Zhengping ; Chow, Hsiao-Hui ; Garcia, Francisco A. / Green tea compound in chemoprevention of cervical cancer. In: International Journal of Gynecological Cancer. 2010 ; Vol. 20, No. 4. pp. 617-624.
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