Growth and water relations of Paulownia fortunei under photomixotrophic and photoautotrophic conditions

P. S. Sha Valli Khan, T. Kozai, Q. T. Nguyen, Chieri Kubota, V. Dhawan

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

The growth and water relations of Paulownia fortunei in photoautotrophic cultures (nutrient medium lacking sucrose and growth regulator) with CO2 enrichment (PWAH) or without CO2 enrichment (PWAL) were compared with those in photomixotrophic shoot (PWC; 30 g dm-3 sucrose and 0.3 mg dm-3 N6-benzyladenine) and root cultures (PWR; 0.3 mg dm-3 indole-3-butyric acid). The photoautotrophic and photomixotrophic cultures were incubated under photosynthetic photon flux 125 and 60 μmol m-2 s-1, respectively. 100 % sprouting and significantly higher number of shoots (1.6) were obtained with PWAH as compared to PWAL and PWC. PWAH and PWAL stimulated spontaneous rooting from the cut end of axillary shoots. In PWAH, 84 % of shoots rooted with an average of 5.9 roots per shoot and 4.0 cm of root length in 21 d. Rooting of photomixotrophic shoot cultures were stimulated by an auxin treatment. In this case, 98.3 % of shoots were rooted with an average of 4.6 roots per shoot and 1.9 cm length. A microscopic observation on leaf abaxial surface prints from photomixotrophic shoot and root cultures showed widely open (6-8 μm) spherical stomata (12-14 μm) and from photoautotrophic cultures elliptical stomata (10-12 μm) with narrow openings (3-4 μm). Leaves from photomixo-trophic cultures had higher stomatal index as compared to photoautotrophic cultures. The rate of moisture loss from detached leaves was not varying significantly in different cultures.

Original languageEnglish (US)
Pages (from-to)161-166
Number of pages6
JournalBiologia Plantarum
Volume46
Issue number2
DOIs
StatePublished - 2003
Externally publishedYes

Fingerprint

Paulownia fortunei
shoots
water
stomata
rooting
sucrose
leaves
indole butyric acid
sprouting
growth regulators
benzyladenine
auxins

Keywords

  • CO enrichment
  • Micropropagation
  • Stomata
  • Water loss

ASJC Scopus subject areas

  • Plant Science

Cite this

Growth and water relations of Paulownia fortunei under photomixotrophic and photoautotrophic conditions. / Sha Valli Khan, P. S.; Kozai, T.; Nguyen, Q. T.; Kubota, Chieri; Dhawan, V.

In: Biologia Plantarum, Vol. 46, No. 2, 2003, p. 161-166.

Research output: Contribution to journalArticle

Sha Valli Khan, P. S. ; Kozai, T. ; Nguyen, Q. T. ; Kubota, Chieri ; Dhawan, V. / Growth and water relations of Paulownia fortunei under photomixotrophic and photoautotrophic conditions. In: Biologia Plantarum. 2003 ; Vol. 46, No. 2. pp. 161-166.
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