Growth factor regulation of bovine satellite cell growth in vitro.

E. A. Greene, Ronald E Allen

Research output: Contribution to journalArticle

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Abstract

This study examined the effects of basic fibroblast growth factor (bFGF), insulin-like growth factor I (IGF-I) and transforming growth factor beta (TGF-beta) on the proliferation and differentiation of primary bovine satellite cells (BSC) in vitro. Individually, these three factors had the following effects on satellite cells: bFGF stimulated proliferation (P less than .01) but inhibited differentiation (P less than .05); IGF-I had no effect on proliferation but stimulated differentiation (P less than .01); and TGF-beta inhibited both proliferation and differentiation (P less than .01). When combined, the following effects were observed: maximum stimulation of proliferation (P less than .01) occurred in the presence of bFGF and IGF-I and differentiation was not stimulated; TGF-beta and bFGF continued to inhibit differentiation (P less than .01), but in the presence of bFGF, TGF-beta stimulated proliferation (P less than .01). No stimulation was observed in the presence of TGF-beta and IGF-I. Bovine satellite cells respond to these three growth factors that have been shown to regulate the activity of other myogenic cells, and in most instances, the responses among cells from various species are similar. These experiments indicate that the interactions of growth factors may be critical in regulating bovine satellite cell activity.

Original languageEnglish (US)
Pages (from-to)146-152
Number of pages7
JournalJournal of Animal Science
Volume69
Issue number1
StatePublished - Jan 1991

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fibroblast growth factor 2
growth factors
transforming growth factor beta
Fibroblast Growth Factor 2
cell growth
Intercellular Signaling Peptides and Proteins
insulin-like growth factor I
Insulin-Like Growth Factor I
Transforming Growth Factor beta
cattle
Growth
cells
Transforming Growth Factor beta1
In Vitro Techniques

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Growth factor regulation of bovine satellite cell growth in vitro. / Greene, E. A.; Allen, Ronald E.

In: Journal of Animal Science, Vol. 69, No. 1, 01.1991, p. 146-152.

Research output: Contribution to journalArticle

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