Growth in serum-free medium of human colonic adenocarcinoma cell lines on microcarriers: A two-step method allowing optimal cell spreading and growth

J. Fantini, J. P. Galons, B. Abadie, P. Canioni, P. J. Cozzone, J. Marvaldi, A. Tirard

Research output: Contribution to journalReview article

12 Scopus citations

Abstract

Human colonic adenocarcinoma cells have been successfully grown on polystyrene microcarriers by modifying the culture conditions used in monolayer culture. The method can be divided into two culture phases: a) a phase of spreading, wherein cells were seeded in presence of serum-supplemented medium; b) a phase of active growth wherein spread cells on the beads were allowed to grow in a serum-free medium. Under these conditions, optimal spreading and growth of HT 29 and HRT 18 cells on the microcarriers were obtained. A differential propagation was observed between HT 29-D4 and HT 29-D9 cells (both clonal populations derived from HT 29 cells) on the microcarriers that is tentatively related to the discrepancy observed in the spreading efficiency of these clonal cells on serum-coated culture flasks. An index of spreading efficiency (IS index) has been defined to quantify the efficiency of spreading of each cell line on microcarriers. These data gave the opportunity to develop serum-free, scale-up methods to culture cells like HT 29 which release potentially useful products.

Original languageEnglish (US)
Pages (from-to)641-646
Number of pages6
JournalIn Vitro Cellular & Developmental Biology
Volume23
Issue number9
DOIs
StatePublished - Sep 1 1987
Externally publishedYes

Keywords

  • HT 29 clones
  • cell culture
  • colonic adenocarcinoma
  • microcarriers
  • serum-free growth

ASJC Scopus subject areas

  • Developmental Biology
  • Clinical Biochemistry
  • Cell Biology

Fingerprint Dive into the research topics of 'Growth in serum-free medium of human colonic adenocarcinoma cell lines on microcarriers: A two-step method allowing optimal cell spreading and growth'. Together they form a unique fingerprint.

  • Cite this