Growth of human rhinovirus in H1-HeLa cell suspension culture and purification of virions

Wai Ming Lee, Yin Chen, Wensheng Wang, Anne Mosser

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

HeLa cell culture is the most widely used system for in vitro studies of the basic biology of human rhinovirus (HRV). It is also useful for making sufficient quantities of virus for experiments that require highly concentrated and purified virus. This chapter describes the protocols for producing a large amount of HeLa cells in suspension culture, using these cells to grow a large quantity of virus of HeLa-adapted HRV-A and -B serotypes, and making highly concentrated virus stock and highly purified virions. These purified HRV virions are free of cellular components and suitable for experiments that are sensitive to cellular contaminations.

Original languageEnglish (US)
Pages (from-to)49-61
Number of pages13
JournalMethods in Molecular Biology
Volume1221
DOIs
StatePublished - Jan 1 2015
Externally publishedYes

Keywords

  • HeLa cells
  • High-titer virus stock
  • Purified virions
  • Suspension culture

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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