Growth-related signaling in vascular smooth muscle cells is deregulated by TCDD during the G0/G1 transition

T. J. Weber, H. F. Fan, R. S. Chapkin, Kenneth Ramos

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Experiments have been conducted to examine the impact of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on growth-related signaling in vascular smooth muscle cells (SMCs). A 40% reduction of peak DNA synthesis was observed in SMCs only when TCDD was added during the G0/G1, transition of the cell cycle. Enhanced phosphorylation of several endogenous proteins during this period was coincident with increased tyrosine kinase activity as early as 15 min following TCDD challenge. No changes in protein phosphorylation status occurred in cells treated with TCDD during the G1/S transition or during S phase. Cotreatment of quiescent SMCs with 10 nM TCDD and serum for 3 h reduced serum-inducible binding activity to a 12-O-tetradecanoyl phorbol 13-acetate responsive element (TRE) by approximately 40%. No alterations of constitutive TRE binding were observed in quiescent SMCs treated with TCDD for up to 5 h. These data show that mitogen-related signaling in vascular SMCs is modulated by TCDD selectively during the G0/G1 transition, and these effects influence the growth behavior of these cells.

Original languageEnglish (US)
Pages (from-to)369-386
Number of pages18
JournalJournal of Toxicology and Environmental Health
Volume51
Issue number4
DOIs
StatePublished - 1997
Externally publishedYes

Fingerprint

Vascular Smooth Muscle
dioxin
Smooth Muscle Myocytes
Muscle
muscle
Cells
Growth
Phosphorylation
Proteins
serum
Period Circadian Proteins
protein
DNA
Tetradecanoylphorbol Acetate
Polychlorinated Dibenzodioxins
1,4-dioxin
Serum
S Phase
Mitogens
Protein-Tyrosine Kinases

ASJC Scopus subject areas

  • Toxicology
  • Pollution
  • Environmental Science(all)
  • Environmental Chemistry
  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis

Cite this

Growth-related signaling in vascular smooth muscle cells is deregulated by TCDD during the G0/G1 transition. / Weber, T. J.; Fan, H. F.; Chapkin, R. S.; Ramos, Kenneth.

In: Journal of Toxicology and Environmental Health, Vol. 51, No. 4, 1997, p. 369-386.

Research output: Contribution to journalArticle

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