GUF1, a gene encoding a novel evolutionarily conserved GTPase in budding yeast

G. L. Kiser, Ted Weinert

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

While sequencing a region of chromosome IV adjacent to the checkpoint gene MEC3, we identified a gene we call GUF1 (GTPase of Unknown Function), which predicts a 586 amino acid GTPase of the elongation factor-type class. The predicted Guf1p protein bears striking sequence similarity to both LepA from Escherichia coli (43% identical) and LK1236.1 from Caenorhabditis elegans (42% identical). Analysis of both a guf1Δ deletion and a putative constitutive-activating mutant (GUF1HG) revealed that GUF1 is not essential nor did mutant cells reveal any marked phenotype. The GenBank Accession Number for the GUF1 sequence is U22360.

Original languageEnglish (US)
Pages (from-to)1311-1316
Number of pages6
JournalYeast
Volume11
Issue number13
DOIs
StatePublished - 1995

Fingerprint

Saccharomycetales
Gene encoding
GTP Phosphohydrolases
guanosinetriphosphatase
Yeast
Genes
yeasts
Peptide Elongation Factors
mutants
Nucleic Acid Databases
Caenorhabditis elegans
Chromosomes
Escherichia coli
Amino acids
Elongation
genes
Proteins
chromosomes
Phenotype
phenotype

Keywords

  • Chromosome IV
  • GTPase
  • GUFI
  • Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Bioengineering
  • Microbiology

Cite this

GUF1, a gene encoding a novel evolutionarily conserved GTPase in budding yeast. / Kiser, G. L.; Weinert, Ted.

In: Yeast, Vol. 11, No. 13, 1995, p. 1311-1316.

Research output: Contribution to journalArticle

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