H2A.F: an extremely variant histone H2A sequence expressed in the chicken embryo.

R. P. Harvey, J. A. Whiting, L. S. Coles, P. A. Krieg, J. R. Wells

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58 Scopus citations


A cDNA clone bank has been constructed from chicken embryonic RNA. Clones hybridizing poorly to embryonic histone gene probes were selected as possible variant gene transcripts. The DNA sequence of one cDNA predicts an extremely variant H2A protein (H2A.F), which is 40% divergent from the most abundant H2A protein in chicken erythrocyte chromatin. The H2A.F gene is not highly conserved across large species barriers, but in the chicken there may be a family of linked genes. The H2A.F mRNA is approximately equal to 820 base pairs in length and, unlike most other histone mRNAs, is polyadenylylated. Significantly, the H2A.F transcript shows a limited tissue distribution in the chicken embryo.

Original languageEnglish (US)
Pages (from-to)2819-2823
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number10
StatePublished - May 1983
Externally publishedYes

ASJC Scopus subject areas

  • General


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