Halting a cellular production line

Responses to ribosomal pausing during translation

John R Buchan, Ian Stansfield

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

Cellular protein synthesis is a complex polymerization process carried out by multiple ribosomes translating individual mRNAs. The process must be responsive to rapidly changing conditions in the cell that could cause ribosomal pausing and queuing. In some circumstances, pausing of a bacterial ribosome can trigger translational abandonment via the process of trans-translation, mediated by tmRNA (transfer-messenger RNA) and endonucleases. Together, these factors release the ribosome from the mRNA and target the incomplete polypeptide for destruction. In eukaryotes, ribosomal pausing can initiate an analogous process carried out by the Dom34p and Hbs1p proteins, which trigger endonucleolytic attack of the mRNA, a process termed mRNA no-go decay. However, ribosomal pausing can also be employed for regulatory purposes, and controlled translational delays are used to help co-translational folding of the nascent polypeptide on the ribosome, as well as a tactic to delay translation of a protein while its encoding mRNA is being localized within the cell. However, other responses to pausing trigger ribosomal frameshift events. Recent discoveries are thus revealing a wide variety of mechanisms used to respond to translational pausing and thus regulate the flow of ribosomal traffic on the mRNA population.

Original languageEnglish (US)
Pages (from-to)475-487
Number of pages13
JournalBiology of the Cell
Volume99
Issue number9
DOIs
StatePublished - Sep 2007
Externally publishedYes

Fingerprint

Messenger RNA
Ribosomes
Ribosomal Frameshifting
Peptides
Endonucleases
Protein Biosynthesis
Transfer RNA
Eukaryota
Polymerization
Proteins
Population

Keywords

  • Frameshifting
  • Peptidyl-tRNA hydrolase
  • Ribosomal drop-off
  • Ribosome
  • Transfer-messenger RNA (tmRNA)
  • Translation

ASJC Scopus subject areas

  • Cell Biology

Cite this

Halting a cellular production line : Responses to ribosomal pausing during translation. / Buchan, John R; Stansfield, Ian.

In: Biology of the Cell, Vol. 99, No. 9, 09.2007, p. 475-487.

Research output: Contribution to journalArticle

@article{28c93182ccfe40f2bf5aedc99009c0c6,
title = "Halting a cellular production line: Responses to ribosomal pausing during translation",
abstract = "Cellular protein synthesis is a complex polymerization process carried out by multiple ribosomes translating individual mRNAs. The process must be responsive to rapidly changing conditions in the cell that could cause ribosomal pausing and queuing. In some circumstances, pausing of a bacterial ribosome can trigger translational abandonment via the process of trans-translation, mediated by tmRNA (transfer-messenger RNA) and endonucleases. Together, these factors release the ribosome from the mRNA and target the incomplete polypeptide for destruction. In eukaryotes, ribosomal pausing can initiate an analogous process carried out by the Dom34p and Hbs1p proteins, which trigger endonucleolytic attack of the mRNA, a process termed mRNA no-go decay. However, ribosomal pausing can also be employed for regulatory purposes, and controlled translational delays are used to help co-translational folding of the nascent polypeptide on the ribosome, as well as a tactic to delay translation of a protein while its encoding mRNA is being localized within the cell. However, other responses to pausing trigger ribosomal frameshift events. Recent discoveries are thus revealing a wide variety of mechanisms used to respond to translational pausing and thus regulate the flow of ribosomal traffic on the mRNA population.",
keywords = "Frameshifting, Peptidyl-tRNA hydrolase, Ribosomal drop-off, Ribosome, Transfer-messenger RNA (tmRNA), Translation",
author = "Buchan, {John R} and Ian Stansfield",
year = "2007",
month = "9",
doi = "10.1042/BC20070037",
language = "English (US)",
volume = "99",
pages = "475--487",
journal = "Biology of the Cell",
issn = "0248-4900",
publisher = "Portland Press Ltd.",
number = "9",

}

TY - JOUR

T1 - Halting a cellular production line

T2 - Responses to ribosomal pausing during translation

AU - Buchan, John R

AU - Stansfield, Ian

PY - 2007/9

Y1 - 2007/9

N2 - Cellular protein synthesis is a complex polymerization process carried out by multiple ribosomes translating individual mRNAs. The process must be responsive to rapidly changing conditions in the cell that could cause ribosomal pausing and queuing. In some circumstances, pausing of a bacterial ribosome can trigger translational abandonment via the process of trans-translation, mediated by tmRNA (transfer-messenger RNA) and endonucleases. Together, these factors release the ribosome from the mRNA and target the incomplete polypeptide for destruction. In eukaryotes, ribosomal pausing can initiate an analogous process carried out by the Dom34p and Hbs1p proteins, which trigger endonucleolytic attack of the mRNA, a process termed mRNA no-go decay. However, ribosomal pausing can also be employed for regulatory purposes, and controlled translational delays are used to help co-translational folding of the nascent polypeptide on the ribosome, as well as a tactic to delay translation of a protein while its encoding mRNA is being localized within the cell. However, other responses to pausing trigger ribosomal frameshift events. Recent discoveries are thus revealing a wide variety of mechanisms used to respond to translational pausing and thus regulate the flow of ribosomal traffic on the mRNA population.

AB - Cellular protein synthesis is a complex polymerization process carried out by multiple ribosomes translating individual mRNAs. The process must be responsive to rapidly changing conditions in the cell that could cause ribosomal pausing and queuing. In some circumstances, pausing of a bacterial ribosome can trigger translational abandonment via the process of trans-translation, mediated by tmRNA (transfer-messenger RNA) and endonucleases. Together, these factors release the ribosome from the mRNA and target the incomplete polypeptide for destruction. In eukaryotes, ribosomal pausing can initiate an analogous process carried out by the Dom34p and Hbs1p proteins, which trigger endonucleolytic attack of the mRNA, a process termed mRNA no-go decay. However, ribosomal pausing can also be employed for regulatory purposes, and controlled translational delays are used to help co-translational folding of the nascent polypeptide on the ribosome, as well as a tactic to delay translation of a protein while its encoding mRNA is being localized within the cell. However, other responses to pausing trigger ribosomal frameshift events. Recent discoveries are thus revealing a wide variety of mechanisms used to respond to translational pausing and thus regulate the flow of ribosomal traffic on the mRNA population.

KW - Frameshifting

KW - Peptidyl-tRNA hydrolase

KW - Ribosomal drop-off

KW - Ribosome

KW - Transfer-messenger RNA (tmRNA)

KW - Translation

UR - http://www.scopus.com/inward/record.url?scp=34548446795&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34548446795&partnerID=8YFLogxK

U2 - 10.1042/BC20070037

DO - 10.1042/BC20070037

M3 - Article

VL - 99

SP - 475

EP - 487

JO - Biology of the Cell

JF - Biology of the Cell

SN - 0248-4900

IS - 9

ER -